野生蝉花与人工蝉花多糖的提取及抑菌试验

Study on the Extraction of Polysaccharide from Wild and Artificial Cordyceps sobolifera and Bacteriostasis Effect

为明确蝉花多糖对食品中常见致病菌的抑制作用,采用微波法从野生蝉花和人工蝉花中提取精多糖,用滤纸片扩散法和比浊法测定多糖的抑菌作用。试验结果表明:两种多糖对大肠杆菌、金黄色葡萄球菌和枯草芽孢杆菌均具有抑制作用,且野生蝉花多糖的抑菌作用较强。

To clarify the bacteriostasis effect to common pathogenic bacteria in the food by the wild Cordyceps sobolifera polysaccharide and the artificial Cordyceps sobolifera polysaccharide the polysaccharides were extracted from the two samples with the microwave and alcohol precipitation, decolorizing, removed protein to make the fine polysaccharide. To determine their polysaccharide content with the phenol-sulphate acid method, the antimicrobial activities of the two polysaccharide were studied with filter paper and nephelometery methods. The results show that both the wild Cordyceps sobolifera and the artificial Cordyceps sobolifera have the bacteriostasis effect to Escherichia coli, Staphylococcus aureus and Bacillus subtilis. And the wild Cordyceps sobolifera has the better function. The lower density, the weaker bacteriostasis effect.