原发性胆汁性肝硬化患者外周血单个核细胞FCGR3A和TYROBP mRNA的表达及其临床意义

Expression of FCGR3A and TYROBP in the Peripheral Blood Mononuclear Cells of Primary Biliary Cirrhosis Patients and Its Clinical Significance

目的通过研究原发性胆汁性肝硬化(primary biliary cirrhosis,PBC)患者外周血单个核细胞(peripheral blood mononuclear cells,PBMC)中免疫球蛋白G Fc段受体Ⅲa(Fc fragment of IgG,low affinity Ⅲ a,receptor,FCGR3A)和酪氨酸激酶结合蛋白(tyrosine kinase binding protein,TYROBP)mRNA的表达,探讨FCGR3A和TYROBP在PBC发病机制中的作用及临床意义。方法采用实时荧光定量逆转录(FQ-RT)-PCR检测80例PBC、55例慢性乙型肝炎(chronic hepatitis type B,CHB)和68名健康对照者(healthy control,HC)PBMC中FCGR3A和TYROBP mRNA基因表达水平,以2-△△Ct法计算基因表达的量,并比较FCGR3A mRNA与TYROBP mRNA表达水平的相关性及FCGR3A和TYROBP mRNA表达水平与肝功能指标的相关性。结果 PBC患者PBMC中FCGR3A mRNA表达水平(1.96±1.87)显著高于疾病对照组CHB患者(1.38±1.19,P=0.044)和HC组(0.75±0.40,P=0.000),CHB患者也明显高于HC组(P=0.000);PBC患者PBMC中TYROBP mRNA表达水平(1.29±0.66)显著高于HC组(0.98±0.36,P=0.009),而与CHB患者无明显差异(1.19±0.76,P=0.233);PBC患者FCGR3A与TYROBP mRNA表达水平有显著相关性(r=0.519,P=0.000);FCGR3A mRNA表达水平与PBC患者肝功能指标r-谷氨酰转肽酶(GGT)的含量呈明显正相关(r=0.427,P=0.000),而与其他指标无相关;PBC患者TYRBOP mRNA表达水平与肝功能指标无相关。结论 PBC患者FCGR3A和TYROBP mRNA表达水平的异常,表明FCGR3A和TYROBP可能参与PBC的发病和疾病的维持。FCGR3A mRNA的表达和肝功能指标GGT相关,可能直接参与PBC肝损伤。

Objective To study the characteristics of Fc fragment of IgG,low affinity Ⅲa receptor（FCGR3A） and tyrosine kinase binding protein（TYROBP） in primary biliary cirrhosis（PBC）,and to analyze the significance of FCGR3A and TYROBP in PBC.Methods mRNA from Peripheral blood mononuclear cells mRNA of 80 PBC patients,55 chronic hepatitis B（CHB） patients and 68 health controls（HC） were detected by fluorescence quantitative reverse transcription poly-merase chain response（FQ-RT-PCR） assay.And the expressions of FCGR3A mRNA,TYROBP mRNA and liver function were compared in PBC patients.Results Expressions of FCGR3A in PBC patients（1.96 ± 1.87） were higher than CHB patients（1.38 ± 1.19,P = 0.044） and HC（0.75 ± 0.40,P = 0.000）.Expressions of TYROBP in PBC patients（1.29 ± 0.66） were higher than HC（0.98 ± 0.36,P = 0.009）,but there was no significant difference between PBC and CHB（1.19 ± 0.76,P = 0.233）.In PBC patients,expression of FCGR3A mRNA was positively correlated with TYROBP mRNA（r = 0.519,P = 0.000） and GGT（r = 0.427,P = 0.000）,but had no correlation with other liver function index.The expression of TYROBP mRNA did not correlate with liver function index.Conclusion Expressions of FCGR3A and TYROBP mRNA significantly elevates in PBC patients,which suggests that these two genes might be involved in the pathogenesis of PBC.In PBC patients,expression of FCGR3A mRNA positively correlates with GGT,which suggests that FCGR3A is directly involved in liver injury.