摘要
目的检测RANTES对类胰蛋白酶引起的肥大细胞白细胞介素-4(interleukin-4,IL-4)分泌和蛋白酶激活受体(protease activated receptor,PAR)-2表达的影响。方法 P815肥大细胞培养后,用不同浓度的RANTES、类胰蛋白酶单独或联合激发肥大细胞,不同时间点收集激发细胞和上清,用酶联免疫吸附试验(ELISA)检测上清中IL-4水平,用流式细胞术检测P815肥大细胞表面PAR-2的表达。结果 RANTES单独作用对肥大细胞IL-4分泌无明显影响,而类胰蛋白酶单独作用则以浓度依赖方式促进肥大细胞IL-4释放(P<0.01);RANTES或类胰蛋白酶单独作用对肥大细胞PAR-2表达均无明显影响(P>0.25)。以RANTES预处理肥大细胞,则类胰蛋白酶促进肥大细胞IL-4释放的作用被显著抑制(P<0.01);而类胰蛋白调节的肥大细胞PAR-2表达显著增强(P<0.01)。结论 RANTES抑制类胰蛋白酶引起的肥大细胞IL-4分泌可能是通过RANTES增强类胰蛋白酶调节PAR-2表达而实现。
Objective This aim of study is to investigate the potential influence of RANTES on tryptase-induced IL-4 release and expression of protease activated receptor(PAR)-2 by using P815 mast cells.Mehtods After P815 mast cells were challenged with different concentrations of RANTES,tryptase alone or combined,the supernatant of challenged cells were analyzed by enzyme-linked immunosorbent assay(ELISA) to detect the release of IL-4,and the challenged cells were collected and analyse by flow cytometry to detect the expression of PAR-2.Results RANTES or tryptase alone elicited little influence on PAR-2 expression of P815 mast cells,whereas pre-incubation of mast cells with RANTES enhanced tryptase-induced PAR-2 expression accordingly.At the same time,RANTES alone had little effect on IL-4 release from P815 mast cells,in contrast tryptase alone promoted IL-4 release from P815 mast cell.But pre-treatment of mast cells with RANTES decreased tryptase-induced IL-4 release from P815 mast cells.Conclusion RANTES could inhibit tryptase-induced IL-4 release from P815 mast cells,which may be related to the modulatory effect of RANTES on tryptase-induced PAR-2 expression.
出处
《中华临床免疫和变态反应杂志》
2010年第4期262-266,共5页
Chinese Journal of Allergy & Clinical Immunology
基金
国家自然科学基金(30772032
30972714
81060250)
海南省自然科学基金(808156)
海南省教育厅科研项目(Hj2009-129)