uPA系统在4株非小细胞性肺癌细胞中的表达

The Expression of u P A Syste m in Four Non small Cell Lung Cancer Cell Line

目的：研究尿激酶型纤溶酶原（ｕ Ｐ Ａ） 激活系统中４ 种主要成分ｕ Ｐ Ａ、 Ｐ Ａ Ｉ１ 、 Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ Ｒ 在非小细胞性肺癌细胞株中的表达及各因子之间的作用方式。方法：应用免疫组化、蛋白免疫印迹技术对培养细胞中４ 种成分进行定位、定性及半定量分析；应用斑点杂交技术检测４ 种细胞中ｕ Ｐ Ａ ｍ Ｒ Ｎ Ａ 和ｕ Ｐ Ａ Ｒｍ Ｒ Ｎ Ａ 的表达。结果：４ 种成分在４ 株细胞的胞浆及胞膜均有表达。蛋白免疫印迹法检测ｕ Ｐ Ａ、 Ｐ Ａ Ｉ１ 、 Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ Ｒ，４ 株细胞均在约１４０ ｋｕ 和约８０ ｋｕ 处出现特异性条带，且两种腺癌细胞的表达水平显著高于其他两株细胞。斑点杂交结果示４ 株细胞中均有ｕ Ｐ Ａ ｍ Ｒ Ｎ Ａ 和ｕ Ｐ Ａ Ｒ ｍ Ｒ Ｎ Ａ 的表达。结论：此４ 株细胞中均能合成与分泌ｕ Ｐ Ａ 系统的４ 种主要成分，各因子以二联及三联复合物（ｕ Ｐ Ａ／ Ｐ Ａ Ｉ１ 、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ２ 、ｕ Ｐ Ａ／ｕ Ｐ Ａ Ｒ、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ１／ｕ Ｐ Ａ Ｒ、ｕ Ｐ Ａ／ Ｐ Ａ Ｉ２／ｕ Ｐ Ａ Ｒ） 的形式存在；ｕ Ｐ Ａ 系统的表达与这４ 种细胞的恶性程度并不一致。

Objective : To study the expression and the reaction patterns ofthe main components ofthe u P Asystem(u P A, P A I 1 , P A I 2 ,u P A R)in non small celllung cancer celllines . Methods :u P Asystem was detected and analyzedusing immunohistochemistry and Western Blot. Dot blot hybridization was used to detect the expression of u P A, u P A Rm R N A. Results : The four components of u P Asystem were expressed in the plasma and membrane ofthe four celllines . Using Western blotto detectthe expression of u P A, P A I 1 , P A I 2 ,u P A R,specific appeared special bands near 80 ku and140 ku were observed , and the expression level of the two adenocarcinoma cell lines was significantly higher than theother two cell lines. Both u P Aand u P A R m R N A were expressed by the four celllines . Conclusions : The four compo nents of u P Asystem could be synthesized and secreted by allthe four celllines ,existed in duplet ortriplet form (u P A/ P A I 1 ,u P A/ P A I 2 ,u P A/u P A R,u P A/ P A I 1/u P A Rand u P A/ P A I 2/u P A R) . The expression of u P Asystem were notinconcomitance with the malignance ofthe celllines .