免疫功能正常小鼠肾囊膜下移植舌癌模型的建立

Establishment of the subrenal capsule model of tongue cancer in normal immunocompetent mouse.

目的：建立免疫功能正常小鼠肾囊膜下移植舌癌模型。材料和方法：利用ＳＲＣＡ 技术，建立移植舌癌模型，观察移植前后瘤体大小的变化，并对移植瘤进行病理切片观察。结果：舌癌移植后第６ 天瘤体增长率为８７．８％ （７／８）（Ｐ＜ ０．０５），病理切片证实其为高分化性鳞癌。结论：小鼠肾囊膜下移植舌癌模型的建立具有可行性，它可为舌癌的基础与临床研究提供一个经济、快速、高效的动物模型。

Objective:To set up a model of tongue cancer that a human tongue xenograft was transplanted under the renal capsule of normal immunocompetent mouse. Materials and Methods: SRCA was used to set up a transplanted model of tongue cancer, and the variation of tumor size was measured between day of sacrifice of the mouse and day of implantation, and the histologic morphology of transplanted tumor was observed. Results:On the 6th day after tongue xenografts were implanted, the growth rate of xenografts was 87.8%(7/8) (t test, P<0.05), and pathology sections proved that the transplanted tumor were highly-differentiate squamous cell carcinoma. Conclusions: It is feasible to set up the mouse subrenal capsule model of tongue cancer, which can provide an inexpensive, rapid, and efficient model for the study of tongue cancer.