反义寡核苷酸阻遏血管平滑肌细胞c-myc,PCNA基因的表达

Study on downregulation of c-myc and PCNA genes expression by antisense oligonucleotides in vascular smooth muscle cells

目的：利用反义寡核着酸阻遏血管平滑肌细胞（VSMC）c-myC，PCNA基因表达，为移植血管再狭窄的基因治疗提供理论依据．方法：人工合成正义、反义及错配反义c-myc，PCNA基因片断，并转人体外培养的VSMC中，运用逆转录聚合酶链式反应（RT-PCR）测定细胞中相应基因表达的情况．结果：反义寡核苦酸能有效抑制VSMC中c-myc，PC-NA基因的表达，而正义和错配反义寡核着酸没有此效应．结论：反义寡核着酸能阻遏VSMC中相应基因的表达．

AIM: To provide the rationale for gene therapy oftransplanted vascular restenosis, antisense oligonucleotideswere applied to attenuate the expression of c--myc and proliferating cell nuclear antigen (PCNA ) genes in vascular smoothmuscle cells (VSMC ). METHODS: The antisense, sense,and mismatched oligonucleotides for c--m pc or PCNA geneswere synthesized and delivered individually to VSMC culturedin vitro. The expression of c--myc and PCNA genes was testedby reverse transcription and polymerase chain reaction (RTPCR) technique. RESULTS: The expression of c--m pc or PCNA genes decreased markedly in the VSMC treated with cmyc or PCNA antisense oligomers, whereas that treated withsense and mismatched oligomers did not. CONCLUSION: caac and PCNA genes antisense oligomers can reduce their related gene expression.