摘要
目的 观察过磷酸化tau蛋白在阿尔茨海默病（AD）患者脊髓中的表达及其在AD发病中的可能机制。方法常规取神经病理证实的3例AD患者脊髓和7例老龄非痴呆性且无重要神经系统疾病对照患者脊髓，胸2、胸8、胸10、腰4和骶2平面取材。常规制片，行Gallyas—Braak染色及过磷酸化tau蛋白（AT8）免疫组织化学染色（SP法），光镜观察过磷酸化tau蛋白在AD及各对照组脊髓的分布情况。结果3例AD患者中2例在脊髓前角出现过磷酸化tau蛋白阳性神经元，3例出现过磷酸化tau蛋白阳性轴索或轴索样结构，3例出现胶质细胞内过磷酸化tau蛋白表达，过磷酸化tau蛋白阳性程度与脑内神经原纤维缠结程度相关；对照组中仅1例脊髓胸2平面可见过磷酸化tau蛋白阳性的点状结构。结论过磷酸化tau蛋白可以在AD患者脊髓内表达，提示轴浆运输障碍可能在AD的发病机制中具有一定作用。
Objective To study the expression of tau-related protein in spinal cord of Chinese patients with Alzheimer＇s disease. Methods Gallays-Braak stain and imnmnohistochemical study for tau protein （ATS） were carried out in the spinal cord tissue （T2, TS, T10, L2 and $2 segments） of 3 Chinese patients with Alzheimer＇s disease. Seven age-matched cases without evidence of dementia or neurologic disease were used as controls. Results Neurofibrillary tangles were identified in the neurons of anterior horn in 2 Alzheimer＇s disease cases but none was observed in the controls. Tau-positive axons and astroglia were detected in all Alzheimer＇s disease cases. Tau immunoreaetivity in spinal cord of the patients correlated with that in brain tissue. Conclusion The expression of tau-related protein is demonstrated in the spinal cord of Alzheimer＇s disease patients suggesting that axonal transport defect may play a role in the pathogenesis of Alzheimer＇s disease.
出处
《中华病理学杂志》
CAS
CSCD
北大核心
2011年第3期161-164,共4页
Chinese Journal of Pathology
基金
国家重点基础研究发展计划（“973”计划）资助项目（2006cb500700）
