黑木耳栽培过程中胞外酶活性变化

Extracellular Enzyme Production by FiveAuricularia auricula Strains at Different Stagesof the Cultivation Cycle

以5个黑木耳(Auricularia auricula)栽培菌株为实验材料,研究了黑木耳栽培过程中4种胞外酶活性的变化。结果表明,不同菌株胞外酶活性不同,但在整个生长发育过程中变化规律基本一致:即随着黑木耳子实体的生长发育,羧甲基纤维素酶(CMCase)和滤纸纤维素酶(FPase)的活性逐渐增强,并在子实体成熟时最高,子实体采收后活性迅速下降;漆酶(LAC)、多酚氧化酶(POD)活性在菌丝生长阶段较高,随着子实体的生长发育酶活下降。

Extracellular polyphenol oxidase（POD）,carboxymethyl cellulase（CMCase）,filter paper cellulase（FPase） and laccase（LAC） production by five Auricularia auricula cultivars was determined at different stages of the cultivation cycle.Although enzyme levels varied among the different cultivars,production patterns were similar in all cases.LAC and POD levels were higher during the substrate colonization phase but declined in the later stages of cultivation.Conversely,CMCase and FPase levels were relatively low during the vegetative growth phase;peak values were recorded at the first fruit body maturation stage（first flush）,and then rapidly decreased.