摘要
以自然衰老小鼠为实验对象,采用邻苯三酚自氧化法测定SOD活性、紫外分光光度法测定CAT活性、愈创木酚法测定POD活性、DTNB直接比色法测定GSH-Px活性,研究了鲤鱼精巢DNA对老龄动物体内抗氧化酶活性的影响。结果表明,鲤鱼精巢DNA可明显提高自然衰老小鼠 体内抗氧化酶的活性。
Effects of carp spermary DNA on various oxidoreductase activities in naturelly senile mice were investigated. The activities of superoxide dismutase (SOD), catalase (CAT), peroxidase (POD) and glutathione peroxidase (GSH-Px) were measured by means of pyrogallol auto-oxidation, ultraviolet spec trometry, guaiacol oxidation and DTNB (5, 5'-dithiobis-2-nitrobenzoic acid) direct colorimetry, respectively. The results showed that DNA from carp spermary could enhance the activities of these enzymes in naturally senile mice.
出处
《中草药》
CAS
CSCD
北大核心
1999年第8期592-594,共3页
Chinese Traditional and Herbal Drugs
关键词
DNA
鲤鱼精巢
抗氧化酶
carp spermary deoxyribonucleic acid (DNA) naturally senile mice Address:Tang Xiaoli, Department of Pharmacology, Sun Yat-sen University of Medical Sciences, Guangzhou