海藻糖联合葡萄糖负载红细胞后溶血效果

Hemolytic effect of trehalose and glucose loading red blood cells

目的:探讨海藻糖和葡萄糖联合负载红细胞的效果,为冷冻干燥红细胞提供新的保存剂。方法:分别采用0、0.125、0.25、0.5和1 mol/L的海藻糖、葡萄糖以及海藻糖联合葡萄糖37℃负载红细胞6 h。然后采用邻甲苯胺法检测上清液中游离血红蛋白含量。结果:负载液浓度为1 mol/L时,3组的细胞溶血程度较重,即上清中游离血红蛋白浓度较高。在浓度低于1 mol/L时,海藻糖联合葡萄糖组与单独海藻糖负载组溶血程度没有明显差别,但是2者低于葡萄糖组。结论:在浓度小于1 mol/L时,海藻糖联合葡萄糖负载红细胞后对细胞的损伤较小,能够满足冻存的负载要求。

Objective：To investigate the hemolytic effects of trehalose and glucose loading red blood cells in order to explore a new preservation agent for lyophilization of red blood cells.Method：The red blood cells were loaded with 0,0.125,0.25,0.5 and 1 mol/L trehalose,glucose and combination of trehalose and glucose respectively in 37℃ for 6 hours.The free hemoglobin concentrations were determined by o-tolidine.Result：Free hemoglobin concentration significantly increased in three groups when the loading solution concentration was 1 mol/L.The changes of free hemoglobin concentrations were not significant in trehalose,combination of trehalose and glucose treatment groups（0.125,0.25,and 0.5 mol/L）,which were both significantly decreased than that of glucose treatment group.Conclusion：Loading with combination of trehalose and glucose would have little hemolytic effect on red blood cells.The combination of trehalose and glucose was qualified for the lyphilization of red blood cells.