摘要
基因型是影响小麦农杆菌转化效率的关键因素。本研究采用携带双元表达载体PCAMB IA-1303的根癌农杆菌菌株AGL 1,对12个"川农"系列小麦品种的幼胚和愈伤进行了遗传转化。GUS瞬时表达的染色结果表明:"川农"系列小麦不同基因型间转化效率差异较大,愈伤组织普遍较幼胚的转化率高,其中幼胚转化效率较高的品种有川农21和川农22,分别为90%和85%;愈伤组织的转化效率普遍高于幼胚,川农22和川农25的愈伤转化率都达到了100%。这些材料可进一步用来转化有益目的基因。
Genotype is the main limiting factor to improve the Agroacterium-mediated wheat transformation efficiency.Twelve Chuannong wheat cultivars were evaluated for the susceptibility to Agrobacterium,by use of strain AGL 1 carrying PCAMBIA-1303.The results from GUS gene transient expression assay showed that transformation rate was quite different among these genotypes.Using immature embryo as explant,the transformation rate of Chuannong 21 and Chuannong 22 were much higher than others,up to 90% and 85% respectively.And the transformation efficiency of callus generally higher than that of immature,among which transformation rate of Chuannong 22 and Chuannong 25 both reached 100%.So,these cultivars could be applied for useful gene transformation.
出处
《种子》
CSCD
北大核心
2011年第3期40-43,共4页
Seed
关键词
“川农”小麦
品种
农杆菌
转化效率
Chuannong wheat
cultivar
agroacterium
transformation efficiency
