5-氟胞嘧啶在体外对转导胞嘧啶脱氨酶基因的肝癌细胞的细胞毒作用

Cytotoxic effects of 5 fluorocytosine on hepatocarcinoma cells transduced with cytosine deaminase gene in vitro

基因治疗为肿瘤治疗开创了一个新途径，其中自杀性基因疗法是肿瘤基因治疗中的一个重要方法．该方法利用复制缺陷型病毒载体将外源基因导入肿瘤细胞，在肿瘤细胞中外源基因表达出酶蛋白，可以将无毒的前体药物转化为细胞毒药物，选择性地杀伤肿瘤细胞．研究表明，自杀性基...

As to investigate the cytotoxic effects of 5 fluorocytosine on hepatocarcinoma cells genetically modified with cytosine deaminase gene, the gene was transduced into the tumour cells with retroviral method. The cytotoxicity and the bystanding killing effects of 5 fluorocytosine on the tumour cells were measured with MTT assay. It was found that the prodrug 5 fluorocytosine had significant cytotoxic effects on human hepatocarcinoma cells which were transduced with cytosine deaminase in vitro. The IC 50 value of 5 fluorocytosine on transgenic and non transgenic hepatocarcinoma cells were 110 and 1 249 mg·L 1 , respectively. Moreover, the result showed that cytosine deaminase gene/5 fluorocytosine system possessed bystander killing effects on hepatocarcinoma. The experiment demonstrates the potential value of the cytosine deaminase gene/5 fluorocytosine system in the treatment of human hepatocarcinoma.