嫁接对盐胁迫下西瓜幼苗生长和可溶性蛋白表达的影响

Effects of grafting on the soluble protein expression in watermelon seedlings under salt stress

采用营养液栽培,以葫芦为砧木,以小型西瓜为接穗,利用SDS-PAGE凝胶电泳,研究了嫁接对50 mmol.L-1 NaCl胁迫下西瓜幼苗生长和可溶性蛋白表达的影响。结果表明:嫁接可使盐胁迫下西瓜幼苗的株高、茎粗、根长、干重、鲜重、叶面积和根系表面积显著增加,叶片可溶性蛋白含量明显提高,大量可溶性蛋白表达增强,并出现247.7×103和107.9×103两种新蛋白;而盐胁迫下自根苗各项生长指标和叶片可溶性蛋白均显著降低,可溶性蛋白表达明显减弱。随着胁迫时间的延长,至少有12种蛋白表达量发生明显变化,其相对分子质量(×103)分别为:244.7、240.0、74.2、61.2、58.1、45.0、42.0、40.1、36.4、29.1、15.7和15.5,这12种蛋白可能与嫁接提高西瓜幼苗耐盐性密切相关。

The effects of grafting on the soluble protein content and expression was conducted with a salt-tolerant Bottle Gourd Peel as the rootstock and a salt-sensitive mini-watermelon as the scion.Results showed that grafting significantly increased the growth and the content and expression level of soluble protein in leaves under 50 mmol·L-1 NaCl stress,while decreased in the own-root seedlings.Two proteins of 247.7×103 and 107.9×103 were expressed only in grafted seedlings,and twelve proteins changed obviously at least,relative molecular weight（×103）were 244.7,240.0,74.2,61.2,58.1,45.0,42.0,40.1,36.4,29.1,15.7 and 15.5.Therefore,it was possible that these proteins have relations with grafting improving the salt tolerance in watermelon seedlings.