鹅胚脾淋巴细胞选择机制研究

Study on the selection mechanism of lymphocytes in goose fetal spleen

取发育到第14天的鹅胚胎(E14)到1日龄雏鹅的脾脏用免疫荧光双标法研究CD4和CD8抗原表达,用Hoeschst33342的荧光染色方法分析细胞凋亡率,探索鹅胚脾淋巴细胞选择机制。结果显示:E14脾初具轮廓,E19脾出现红髓和白髓,1日龄时脾被膜增厚,脾小结数目增多,脾结构完整。CD4和CD8共表达于脾的红髓和被膜上,白髓中表达量较少。鹅胚脾的CD4+CD8+表达量为1%～30%;1日龄雏鹅的双阳性面积百分比与胚胎鹅E14、E19、E24、E27差异极显著(P<0.01);1日龄雏鹅的凋亡率与胚胎鹅E14、E24差异极显著(P<0.01)。表明鹅胚脾的CD4+CD8+表达与细胞凋亡存在协同关系。

The selection mechanism of lymphocytes of the fetal spleen at the age from E14（14-day embryos）to 1 day-old（1 d after hatching）were studied by morphological observation,and the expression of CD4 and CD8 was studied using the method of double-labeled immunofluorescence,and the apoptosis was also analyzed with the fluorescent dye Hoeschst 33342.The results were shown as follows：the spleen formed until the spleen at E14.The red and white pulp appeared at E19.The spleen capsule became thicker at 1 day-old.The cell number in splenic corpuscle increased,and the spleen structure grew mature.CD4＋CD8＋ immunofluorescence was distributed in the splenic red pulp and the membrane,but absent in white pulp.In goose embryo,the expression of CD4＋CD8＋ was around from 1% to 30%.Compared with 1 day-old,the expression of CD4＋CD8＋ of E14,E19,E24 and E27 was significantly different（P〈0.01）;Compared with 1 day-old,the apoptotic rate of E14 and E24 was significantly different（P〈0.01）.It was suggested that there was a coordination relationship between expression of CD4＋CD8＋ and apoptosis in the spleen of the goose.