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应用PCR-DGGE指纹图谱技术分析盐水鸭贮藏过程中的细菌多样性 被引量：7

Bacterial diversity in water-boiled salted duck during storage analyzed by PCR-DGGE
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摘要 利用传统平板培养和变性梯度凝胶电泳(DGGE)指纹图谱相结合的方法,对盐水鸭在4℃贮藏期间的细菌多样性进行了分析。传统培养方法结果显示:盐水鸭在贮藏期间细菌数量增加迅速。PCR-DGGE对盐水鸭及平板培养物中的细菌组成分析结果显示:腐生葡萄球菌(Staphylococci saprophyticus)、溶酪大球菌(Macrococcus caseolyticus)、魏斯氏菌(Weissellasp.)、中度嗜盐菌(Halomonas sp.)和盐单胞菌(Cobetia sp.)是盐水鸭的主要细菌,而腐生葡萄球菌、溶酪大球菌和魏斯氏菌是平板培养物中的主要细菌。 Traditional plate culture and polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE）methods were used to investigate the bacterial diversity in water-boiled salted duck during storage at 4 ℃.The results of the plate culture showed that bacterial communities in MRS and PCA agar plate increased quickly during storage.The analysis of PCR-DGGE patterns showed that Staphylococci saprophyticus,Macrococcus caseolyticus,Weissella sp.,Halomonas sp.and Cobetia sp.were the main bacteria in water-boiled salted duck,while S.saprophyticus,M.caseolyticus and Weissella sp.were the main bacteria in plate culture agar.
作者 刘芳 王道营 徐为民 诸永志 曹建民
机构地区 江苏省农业科学院农产品加工研究所
出处 《南京农业大学学报》 CAS CSCD 北大核心 2011年第2期135-138,共4页 Journal of Nanjing Agricultural University
基金 江苏省自然科学基金项目(BK2010469) 江苏省农业自主创新基金项目(CX(10)440)
关键词 盐水鸭 细菌多样性 16SrDNA 变性梯度凝胶电泳 water-boiled salted duck bacterial diversity 16S rDNA denaturing gradient gel electrophoresis
分类号 TS251.1 [轻工技术与工程—农产品加工及贮藏工程]
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参考文献9

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共引文献24

同被引文献125

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引证文献7

二级引证文献46

南京农业大学学报
2011年 第2期

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