大型海藻无性系微球体构建和反应器培养研究

Studies on developments of microspheres from macroalgal clones and cultivations in airlift photobioreactor

利用大型海藻细胞的全能性获得了蜈蚣藻(Grateloupia filicina)、多管藻(Polysiphonia urceolata)和孔石莼(Ulva pertusa Kjellm)的无性系材料,在此基础上分别构建了绒球状微球体、网状微球体和簇生状微球体3种微球体形式,这种微球体形式适合于大型海藻的生物反应器高密度培养。簇生状的孔石莼微球体在10 L藻类生物反应器中的培养密度达5.81 g/L鲜质量,最高生长速度达到0.36 g/L/d鲜质量,最大比生长速率为0.103/d。藻类生物反应器高密度培养海藻无性系可用于海藻育苗、水产养殖水处理以及天然活性物质生产等。

Three macroalgal clones of Grateloupia filicina,Polysiphonia urceolata and Ulva pertusa,respectively,were obtained by the totipotency of marine algae.From them,three types of algal microspheres suitable for cultivation in a 10-L airlift photobioreactor were developed,which were flossy,netty and fascicular,respectively.The tufted microspheres of U.pertusa were cultivated in the photobioreactor to the biomass density of 5.81 g/L FW L,with the maximum growth rate of 0.36 g/（L d）FW and specific growth rate of 0.103 d 1.The technology of high-density culture of macroalgae in photobioreactor will have wide applications,including seeding,mass cultivation,integrate-aquaculture,and manufacture of bioactive products.