人股动脉粥样硬化相关蛋白的鉴定及其生物学作用

Proteomics analysis on pathogenesis of human atherosclerosis obliterans in human femoral artery

目的应用比较蛋白质组学方法探讨人动脉粥样硬化闭塞症（ASO）差异蛋白质的表达及其在ASO发病机制中的作用。方法选取ASO股动脉8例及正常股动脉5例，提取组织蛋白质，行双向凝胶电泳分离、质谱分析及数据库查询，获得差异蛋白信息。结果成功建立ASO和正常股动脉双向电泳图谱，含量相差大于2倍以上的蛋白质53个，27个上调，26个下调，质谱鉴定出13种蛋白质，主要与炎症、免疫、氧化应激、脂质代谢等相关。结论ASO与正常股动脉蛋白质组明显差异，差异蛋白质可能在ASO中起重要作用。

Objective We used proteomie profiling in an attempt to differentiate and identify histological proteins that were associated with atheroselerosis obliterans （ASO） of human femoral artery. Methods We comparatively analyzed the proteome of 8 atherosclerotic and 5 normal femoral arteries. The differentially expressed proteins were visualized by two dimensional electrophoresis （2-DE） and sequenced by matrix assisted laser desorption/ionization mass spectrometry （MALDI-TOF-MS）. The protein identifi- cation program was used to search the National Center for Bioteehnology Information （NCBI） database and International Protein Index （IPI）. Results A total of 53 distinct spots corresponding to 13 different proteins were identified by MALDI-TOF-MS using the NCBI and IPI databases. The function information of these 13 proteins mainly involved the pathogenetic mechanisms such as inflammation, innate immunity, oxidative stress, lipid metabolism, amyloid degeneration and so on. Conclusion ASO is associated with dis- tinct patterns of protein expression in the femoral arteries, and differentially expressed 13 proteins may con- tribute to the pathogenesis. These findings might provide needed biomarkers for ASO and new insight into its pathophysiology.