单核／巨噬细胞在下肢静脉性溃疡的表达

Expression of monocyte/macrophage in venous ulceration of lower limbs

目的探讨单核／巨噬细胞在下肢静脉性溃疡的表达及其意义。方法将下肢慢性静脉功能不全患者28例分为溃疡组和无溃疡组。采用血细胞计数、流式细胞技术、逆转录一聚合酶链反应（RT—PCR）和免疫组织化学方法检测不同体位下肢外周血和局部皮肤单核／巨噬细胞的表达。另选10例健康志愿者为对照组。结果各组间两两比较，下肢外周血单核细胞计数和CD68阳性细胞率比较差异无统计学意义（P〉0．05）；溃疡组平卧位、下垂位血液中CD68阳性单核／巨噬细胞mRNA相对值分别为（0．81±0．21）和（0．66±0．24）；皮肤中阳性细胞数为（60．31±14．20）、（58．10±16．23）；与无溃疡组和对照组比较，差异均有统计学意义（P〈0．05）；各组间不同体位之间单核／巨噬细胞免疫组织化学胞阳性细胞数和含量比较，差异无统计学意义（P〉0．05）。结论单核／巨噬细胞浸润与下肢静脉性溃疡的发生密切相关。

Objective To investigate the expression and significance of monocyte/macrophage in venous ulceration. Methods Twenty-eight cases of chronic venous insufficiency were divided into ulcera- tion group, non-ulceration group and control group. The expression of monocyte/macrophage was analyzed by blood routine, flow cytometry, reverse transcription-polymerase chain reaction （RT-PCR） and immuno- histochemistry in different positions. Ten health volunteers served as controls. Results There was no sig- nificant difference in blood monocyte/macrophage among groups. In ulceration group, monocyte/macro- phage mRNA： （0. 81 ±0. 21 ）, （0. 66 ± O. 24）, and the positive cells in skin were （60. 31 ± 14.20）, （58.10 ± 16. 23）, showed significant difference as compared with the other two groups （P 〈0.05）. There was no significant difference between different positions （ P 〉 0.05 ）. Conclusion There is a close corre- lation between monocyte/macrophage infiltration and venous ulceration of lower limbs.