肿瘤转移相关基因在原发性肝细胞癌中的表达及其临床意义

Expression of metastasis associated-1 in human original hepat ocellular carcinoma and its clinical significance

目的观察肿瘤转移相关基因（MTAl）在原发性肝细胞癌（HCC）中的表达，探讨MTA1与HCC浸润和转移的关系。方法分别采用免疫组织化学sP法和逆转录-聚合酶链反应（RT—PCR）方法检测42例HCC、10例肝硬化和7例正常肝组织内MTA-1的表达，并结合临床病理指标进行分析。结果MTAl在HCC、肝硬化和正常肝脏组织中的阳性例数分别为26、2和0例，差异有统计学意义（P〈0．05）；HCC组织内MTAlmRNA的表达强度明显高于肝硬化组织（t=2．792，P〈0．05）；MTAl在有包膜侵犯组、病理分级Ⅲ～Ⅳ组及有转移HCC中的阳性表达率及强度明显高于无包膜侵犯组（P〈0．05）、病理分级I～Ⅱ级组（P〈0．05）以及无转移组（P〈0．01）。结论MTAl在HCC组织中的表达上调与HCC的浸润和转移有关。

Objective To study the expression of metastasis associated-1 （ MTA1 ） in human hep- atocellular carcinoma （HCC） and its relationship with invasion and metastasis of HCC. Methods By u- sing the method of immohistochemical staining （SP） and reverse transcription-polymerase chain reaction （RT-PCR） , specimens from 42 cases of HCC, 10 cases of hepatic cirrhosis （HC） and 7 normal liver tis- sues （NL） were studied. The relationship between the MTA1 expression and HCC pathological features was evaluated. Results MTA1 positive cases in the HCC, HC and NL tissues was 26, 2 and 0 respectively, with the significant difference between HCC and HC as well as NL （ P 〈 O. 05 ）. The expression of MTA1 mRNA in HCC tissues was significantly higher than that in HC （ t = 2. 792, P 〈 O. 05 ）. Overexpression of metastasis associated-1 in the HCC was related to the invasion of tumor envelop （ P 〈 0. 05 ） , Edmondson grades （P 〈 0. 05 ） and tumor metastasis （P 〈 0. 01 ）. Conclusion MTA1 may play an important role in invasion and metastasis of HCC.