乙型肝炎病毒相关肝细胞癌中的肝癌相关mRNA的筛选和分析

Preliminary screening and analysis on mRNA relating to liver cancer in HBV-associated hepatocellular carcinoma

目的观察乙型肝炎病毒（HBV）相关肝细胞癌的mRNA表达谱的变化，并进行生物信息学分析。方法用长链RNA芯片检测肝癌组织和正常肝组织mRNA的表达谱，并进行GO分析将差异表达的基因进行功能和细胞定位。结果肝癌mRNA表达量与正常对照组比较，2倍以上变化，差异有统计学意义（P〈0．05）的mRNA认为是差异表达的mRNA，共991条，占所有基因总数的5．7％。其中2倍以上升高的共733条；2倍以上降低的共258条；5倍以上升高的共94条；5倍以上降低的共39条；10倍以上升高的共18条；10倍以上降低的共11条。GO分析表明差异性表达的mRNA参与了重要生物学调节功能，其中一些基因可能是HBV相关肝癌所特有的。结论HBV相关肝癌的mRNA表达谱发生显著变化，差异基因参与了细胞的重要生物调节过程，可能参与肝癌的重要调节过程；HBV相关肝癌的表达谱与其他病因相关的肝癌有共性，也有一定的特性。

Objective To explore mRNA expression profile variation of hepatitis B virus （HBV） - related hepatocellular carcinoma and analyze bioinforulatics. Methods Long-chain RNA chip was used to inspect mRNA expression profile in HBV-related hepatocellular carcinoma and normal hepatic tissue and make GO analysis for function and cellular localization of genes with differential expression. Results By comparing mRNA expression level of liver cancer with normal control group, 991 mRNA which had more than 2 times variation and significant difference （P 〈 0. 05） by statistical analysis were regarded as differ- ential expression mRNA, accounting for 5.7% of all genes. 733 mRNA were increased by more than 2 times and 258 decreased by more than 2 times; 94 increased by more than 5 times, 39 decreased by more than 5 times; 18 increased by more than 10 times, 11 decreased by more than 10 times. GO analysis re- vealed that differential expression mRNA involved in significant biological regulatory function, and some genes may be particular to HBV-related liver cancer. Conclusion The mRNA expression profile in HBV- related liver cancer changes significantly, and the different genes participate in significant biological regula- tion process of cells and maybe participate in significant regulation process of liver cancer; HBV-related liv- er cancer＇ s expression profile has certain characteristics and something in common with liver cancers rela- ting to other pathogenesis.