摘要
目的通过两种方法构建兔VX-2移植性原位。肾癌模型,并对其进行评估。方法将30只新西兰白兔随机分2组,每组15只。A组采用开放手术下VX-2肿瘤组织块包埋种植法建模;B组采用经皮超声引导下VX-2肿瘤组织悬液经皮注射种植法建模,通过连续的CT及病理观察与评估模型的稳定性。结果两组建模均成功。A组模型所有肿瘤均为单发,呈外生性肿瘤,形状规则。B组模型有2只出现腹壁种植,占13.3%,肾脏肿瘤生长多形状不规则,常较早突破包膜,沿人针处向肾外生长,部分肾脏肿瘤为完全内生型肿瘤,早期即侵犯集合系统。结论采用开放手术下VX-2肿瘤组织块包埋种植法建模,肿瘤具有单发、呈外生性生长、形状规则等特点,有利于肾癌如射频消融等微创治疗的实验研究,为其提供稳定的动物模型。
Objective To establish a model of implanted renal carcinoma in situ and compare the advantages and disadvantages of two methods to establish the rabbit VX-2 renal carcinoma model. Methods Thirty purebred New Zealand white rabbits were divided evenly and randomly into two groups and the VX-2 renal carcinoma model was established. In group A tumor block was implanted by open operation, and in group B VX-2 tumor tissue suspension was subcutaneously injected under the guide of ultrasound. Results Tumor was successfully implanted in both groups. In group A, tumor grew in proper shapes and posi- tions. In group B there were two abdominal implanting examples ( 13.3% ), and tumor usually grew out- side the kidney throughout the pin hole or even encroached on the kidney collecting system in the first two weeks. Conclusion The tumor tissue block implanting can be used as a better method than the tumor tissue suspension injection in modeling the VX-2 renal carcinoma in situ.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第4期634-636,F0004,共4页
Chinese Journal of Experimental Surgery
基金
基金项目:江苏省卫生厅课题资助项目(Z200712)
南京市卫生局一般项目(YKK08060)
南京市科技发展计划资助项目(201001104)
关键词
兔
VX-2肾癌
模型
动物
Rabbit
VX-2 renal carcinoma
Model,animal