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兔VX-2原位肾癌模型的建立 被引量:3

Implanted VX-2 rabbit renal cell carcinoma in situ
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摘要 目的通过两种方法构建兔VX-2移植性原位。肾癌模型,并对其进行评估。方法将30只新西兰白兔随机分2组,每组15只。A组采用开放手术下VX-2肿瘤组织块包埋种植法建模;B组采用经皮超声引导下VX-2肿瘤组织悬液经皮注射种植法建模,通过连续的CT及病理观察与评估模型的稳定性。结果两组建模均成功。A组模型所有肿瘤均为单发,呈外生性肿瘤,形状规则。B组模型有2只出现腹壁种植,占13.3%,肾脏肿瘤生长多形状不规则,常较早突破包膜,沿人针处向肾外生长,部分肾脏肿瘤为完全内生型肿瘤,早期即侵犯集合系统。结论采用开放手术下VX-2肿瘤组织块包埋种植法建模,肿瘤具有单发、呈外生性生长、形状规则等特点,有利于肾癌如射频消融等微创治疗的实验研究,为其提供稳定的动物模型。 Objective To establish a model of implanted renal carcinoma in situ and compare the advantages and disadvantages of two methods to establish the rabbit VX-2 renal carcinoma model. Methods Thirty purebred New Zealand white rabbits were divided evenly and randomly into two groups and the VX-2 renal carcinoma model was established. In group A tumor block was implanted by open operation, and in group B VX-2 tumor tissue suspension was subcutaneously injected under the guide of ultrasound. Results Tumor was successfully implanted in both groups. In group A, tumor grew in proper shapes and posi- tions. In group B there were two abdominal implanting examples ( 13.3% ), and tumor usually grew out- side the kidney throughout the pin hole or even encroached on the kidney collecting system in the first two weeks. Conclusion The tumor tissue block implanting can be used as a better method than the tumor tissue suspension injection in modeling the VX-2 renal carcinoma in situ.
出处 《中华实验外科杂志》 CAS CSCD 北大核心 2011年第4期634-636,F0004,共4页 Chinese Journal of Experimental Surgery
基金 基金项目:江苏省卫生厅课题资助项目(Z200712) 南京市卫生局一般项目(YKK08060) 南京市科技发展计划资助项目(201001104)
关键词 VX-2肾癌 模型 动物 Rabbit VX-2 renal carcinoma Model,animal
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参考文献6

  • 1Kigure T, Harada T, Yuri Y, et al. Ultrasound-guided microwave thermotherapy on a VX-2 carcinoma implanted in rabbit kidney. Ultrasound Med Biol, 1995,21:649.
  • 2Jerde TJ, Johnson DC, Lee FY, et al. Efficacy of therapeutic techniques for implanted VX-2 carcinoma in rabbit kidneys: comparison between radiofrequency, cryoablation, and nephrectomy. Uro1,2000,163 : 8.
  • 3Zhang DK, Dong BW, Wang Y, et al. Percutaneous microwave ablation or nephrectomy for VX-2 carcinoma in rabbit kidney. Urol 2009,182: 1588-1593.
  • 4Carlsson G, Gullerg, B, Hastroml F, et al. Estimation of liver tumor volume using different formulas: an experimental study in rats. Cancer Res Oncol, 1983,105:20-23.
  • 5Wang D, Bangash AK, Rhee TK, et al. Liver tumors : monitoring embolization in rabbits with VX-2 tumors-transcatheter intraarterial firstpass per'fusion MR imaging. Radiology,2007,245:130-139.
  • 6Chen JH, Lin YC, Huang YS, et al. Induction of VX2 carcinoma in rabbit liver:comparison of two inoculation methods. Lab Anim,2004, 38:79-84.

同被引文献19

  • 1谭晓洁,侯建国,贺松琴,常文军,刘世建,余永伟,曹广文.人肾细胞癌原位移植裸鼠模型的建立及转移相关基因表达分析[J].第二军医大学学报,2006,27(3):263-267. 被引量:3
  • 2邢雪,吴在德,陈孝平,沈关心.肝癌患者免疫功能的临床研究[J].中华实验外科杂志,1996,13(6):333-334. 被引量:23
  • 3MeDougal WS, Gervais DA, McGovern FJ, et al. Long-term followup of patients with renal cell carcinoma treated with radio frequency abla- tion with curative intent. J Urol, 2005,174:61-63.
  • 4Rughetti A, Rahimi H, Rossi P, et al. Modulation of blood circulating immune cells by radiofrequency tumor ablation. J Exp Clin Cancer Res, 2003,22 : 247-250.
  • 5Raj GV, Reddan D, Hoey MF, et al. Management of the small renal umor with radlofrequency ablation. Urology ,2003,61:23-29.
  • 6Binah O. Cytotoxic lymphocytes and cardiac electrophysiology. J Mol Cell Cardio1,2002,34 : 1147-1161.
  • 7Liua Y, Poona RT, Hughesb J, et al. Chemokine receptors support in- filtration of lymphocyte subpopulations in human hepatocellular carci- noma. Clin Immunol, 2005,114 : 174-182.
  • 8den Brok MH,Sutmuller RP,van der Voort R,et aL In situ tumor ab- lation creates an antigen source for the generation of antitumor immu- nity. Cancer Res,2004,64:4024-4029.
  • 9Liu GJ,Moriyasu F, Hirokawa T, et al. Expression of heat shock pro- tein 70 in rabbit liver after contrast-enhanced ultrasound and radiofre- queney ablation. Ultrasound Med Bio1,2010,36:78-85.
  • 10Ostberg JR,Taylor SL, Baumann H, et al. Regulatory effects of fever- range whole body hyperthermia on the LPS-induced acute inflammato- ry response. J Leukoc Biol,2000,68 : 815-820.

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