葛根素对糖尿病大鼠心肌细胞凋亡及相关蛋白表达的影响

Effects of Puerarin on myocardial cell apoptosis and related protein expression in diabetic rats

目的:探讨葛根素(Puerarin,Pue)对糖尿病大鼠心肌细胞凋亡及相关蛋白表达的影响。方法:40只SD大鼠随机分成空白组、糖尿病模型组、Pue低剂量组和Pue高剂量组各10只。对4组大鼠行空腹血糖、心肌细胞凋亡指数(AI)、免疫组化和Western-Bloting检测。结果:Pue各治疗组均较模型组有显著意义改变:空腹血糖降低;AI下降;Bcl-2表达升高,Caspase 3和Cyt-C表达均下降;且高剂量组的改善情况更为理想。结论:Pue可以改善糖尿病大鼠空腹血糖和AI,其机制可能与调节Bcl-2、Caspase 3和Cyt-C水平有关。

Objective：To investigate the effects of Puerarin on myocardial cell apoptosis and related protein expression in diabetic rats.Methods：The forty SD rats were randomly divided into four groups：the control group,the diabetic model group,the Puerarin low-dose group and high-dose group.Rats were respectively measured fasting blood glucose,apoptosis index（AI）,immunohistochemistry and Western Blot.Results：Puerarin therapy groups than model group are statistically significant improvement：fasting blood glucose and AI were decreased,Bcl-2 expression in turn is increased,Caspase 3 and Cyt-C expression are decline in turn,and the high doses group improvement situation is most ideal.Conclusion：The expression of Bcl-2、Caspase 3 and Cyt-C are related to diabetic rats,Puerarin has protective effect on blood glucose and AI through affecting the changes of above mentioned indexes.