摘要
目的研究曲古抑菌素A(TSA)对人胃癌细胞的增殖、凋亡及bcl-2、caspase-3 mRNA表达的影响及其意义。方法体外培养人胃癌细胞株SGC-7901,MTT法检测不同浓度TSA作用不同时间后细胞的增殖情况;流式细胞术检测不同浓度TSA作用48 h后的细胞凋亡情况;RT-PCR检测胃癌细胞中bcl-2、caspase-3 mRNA表达情况。结果不同浓度TSA作用不同时间后,随着时间延长及浓度增高,细胞的增殖抑制明显,呈现明显时间-效应关系及剂量效应关系;与阴性对照组比较,随着TSA浓度的增加,胃癌细胞SGC-7901的早期凋亡率逐渐增高,差异有统计学意义(P<0.05);与阴性对照组比较,胃癌细胞中bcl-2 mRNA随TSA浓度的增高,表达逐渐下调,而caaspase-3mRNA随TSA浓度的增高表达逐渐上调,差异有统计学意义(P<0.05)。结论 TSA对人胃癌细胞SGC-7901具有明显生长抑制作用,呈时间与剂量依赖性;能诱导人胃癌细胞SGC-7901发生早期凋亡能诱导胃癌细胞株SGC-7901细胞发生早期凋亡;TSA抑制人胃癌细胞SGC-7901增殖与诱导凋亡作用可能与bcl-2 mRNA表达下调、caspase-3mRNA表达上调有关。
Objective To investigate the effect of trichostain A（TSA） on growth and expression of bcl-2,caspase-3 mRNA and growth of human gastric cancer cell line SGC-7901 in vitro,and to explore its significance.Methods SGC-7901 human gastric cancer cells were cultured in vitro.MTT colorimetric assay was used to determine the proliferation rate of cells,when SGC-7901 cells were cultured in medium containing the TSA in different time and concentrations.The flow cytometry（FCM） method was used to study the apoptosis and cell cycle induced by TSA in SGC-7901 cells.The expression of bcl-2 and caspase-3 mRNA was determined by reverse transcriplase polymerase chain reaction（RT-PCR）.Results MTT assay indicated that the inhibitory effect of TSA on the proliferation of SGC-7901 cells was in does-and-time dependent manner.Comparing with the control group,the cell apoptosis was induced by TSA at 48 hours and increased in does-dependent manner（P0.05）.RT-PCR assay showed that the expression of bcl-2 mRNA was downregulated and caspase-3 mRNA was upregulated by TSA at 48 hours with concentration increase（P0.05）.Conclusion The growth of SGC-7901 cell in vitro is inhibited by TSA and in dose-and-time dependent manner.TSA also can induce cell apoptosis.Maybe its mechanism is through downgulating the expression of bcl-2 mRNA,upregulating the expression of caspase-3 mRNA.
出处
《苏州大学学报（医学版）》
CAS
北大核心
2010年第6期1197-1200,共4页
Suzhou University Journal of Medical Science
