Straw-top玻璃化与程序化冷冻小鼠8-细胞胚胎效果的比较

Comparison of Slow-freezing and Straw-top Vitrification Method for 8-cell Mouse Embryos Cryopreservation

目的比较慢速程序化法与玻璃化法冷冻小鼠8-细胞胚胎的效果。方法将小鼠卵母细胞行体外受精后培养至8-细胞,分别采用慢速程序化法与玻璃化法进行冷冻,解冻后比较两组胚胎存活率及囊胚形成率。结果慢速程序化冷冻法解冻后的胚胎存活率为79.6%,囊胚形成率为70.7%;玻璃化冷冻法解冻后的胚胎存活率为94.9%,囊胚形成率为89.9%,胚胎存活率与囊胚形成率均显著高于前者(均P<0.01)。结论在进行小鼠8-细胞胚胎冷冻时,玻璃化法冷冻效果较慢速程序化法效果好。

Objective To compare slow-freezing and vitrification method for 8-cell mouse embryos cryopreservation.Methods The mouse 8-cell embryos derived from in vitro fertilization were cryopreserved with slow-freezing and vitrification method.Then the embryos were thawed to observe cryosurvival rate and blastocyst formation rate.Results The survival rate and blastocyst formation rate of embryos frozen with vitrification method were 94.9% and 89.9%,which was significantly higher than those with slow-freezing method 79.6% and 70.7%（all P0.01）.Conclusion The 8-cell mouse embryos could be more effectively cryopreservated with vitrification than those with slow-freezing.