川崎病患儿外周血中FOXP3 mRNA表达与CD4^+CD25^+调节性T细胞的变化

Changes of Expression of FOXP3 mRNA and Proportion of CD4^+CD25^+ Regulatory T Cells in Children with Kawasaki Disease

目的探讨CD4+CD25+调节性T细胞在川崎病(KD)发病机制中作用。方法 32例KD患儿为研究对象,以年龄匹配的30名儿童作为对照组(其中15例急性呼吸道感染的发热患儿为非KD发热组,15名健康体检儿童为健康组),KD患儿分别于静注丙种球蛋白(IVIG)治疗前和静注IVIG治疗后热退2～3 d取外周血用流式细胞仪测CD4+CD25+调节性T细胞,并用RT-PCR测定外周血单个核细胞(PBMC)叉头/翼状螺旋转录因子(FOXP3)mRNA的表达水平。结果急性期KD患儿外周血CD4+CD25+调节性T细胞比例与对照组相比均显著降低(P<0.01),而IVIG治疗后热退2～3 d,其比例显著升高,接近于正常水平,而外周血PBMC FOXP3 mRNA表达水平也出现同样的改变。结论 CD4+CD25+调节性T细胞可能参与了KD的发病机制。

Objective To investigate the role of CD4 ＋CD25 ＋ regulatory T cells in the pathogenesis of Kawasaki disease（KD）.Methods Thirty-two patients with KD and thirty age-matched control subjects（15 febrile patients with common infection disease and 15 healthy children） were included in the study.Real time PCR（RT-PCR） was applied to detect the expression of FOXP3 mRNA,and flow cyto-meter（FCM） was used to determine the proportion of CD4 ＋CD25 ＋ regulatory T cells.All patients with KD in this study were detected fot two times [before the intravenous immunoglobulin（IVIG） treatment and 2~3 days after relief of high fever due to IVIG treatment].Results Compared with febrile patients with common infection disease（mean 6.37%） and healthy children（mean 7.45%）,the proportion of CD4 ＋CD25 ＋regulatory T cells in the KD children at acute phase（mean 4.40%） decresed significantly（P0.01）,while in the defervescence phase,it increased obviously（mean 6.88%）.The same results were found when it was cerned with the FOXP3 mRNA expression.Conclusion CD4 ＋CD25 ＋ regulatory T cells may take part in the pathogenesis of KD.