ATRA在卵巢癌细胞H08910增殖中的作用

THE EFFECT OF ATAR IN THE PROLIFERATION OF HUMAN OVARIAN CANCER CELLS HO8910

目的：探讨全反式雏甲酸在人卵巢癌细胞生长和增殖中的作用机制。方法：选用人卵巢癌细胞株H08910在体外进行培养，培养时添加不同浓度的全反式维甲酸（all—tralns retinoie acid，ATRA），作用24h、48h、72h后，采用MTT比色法测定细胞生长抑制率；采用流式细胞术（flow cytometry，FCM）进行细胞周期分析；采用RT—PCR法检测维甲酸仅受体（retinoic acid receptorct，RARer）mRNA的表达。结果：不同浓度的全反式雏甲酸对H08910细胞生长均起到抑制作用（P〈0．05），并呈浓度和时间依赖关系；能够增加G1期细胞比例，同时降低S、G2／M期细胞比例（P〈0．05），细胞增殖指数PI（P〈0．05）降低；能够上调雏甲酸α受体mRNA的表达（P〈0．05），从而抑制卵巢癌细胞增殖。结论：说明全反式维甲酸对卵巢癌细胞H08910的增殖具有显著的抑制作用，其机制可能是通过上调维甲酸α受体mRNA的表达来实现的。

Objective： To study the effect of all - trans retinoic acid （ATRA） in the proliferation of human ovarian cancer cells. Methods： HO8910 human ovarian cancer cell lines were cultured in vitro and treated with different concentrations of all - trans retinoic acid for 1,2 or 3 days. Cell proliferation was evaluated by MTT assay. Cell cycle was analyzed by flow cytometry （FCM）. The expres- sion of retinoic acid receptor α mRNA was detected by reverse transcription - polymcrase chain reac- tion （RT- PCR）. Results： Different concentrations of all -trans retinoic acid could inhibit the growth of HO8910 cells（P 〈0.05） in a time and dose - dependent manner. All - trans retinoic acid increases the rates of G1 and decreases the rates of S and G2/M （ P 〈 0.05 ）, and therefore, it decreased cells proliferation index （PI） （P 〈 0.05 ）. It could up - regulate the expression of retinoic acid receptor α mRNA（P 〈 0.05）, then inhibit proliferation of H08910 cells. Conclusion：all- trans retinoic acid could inhibit the proliferation of human ovarian cancer cells H08910, the molecular basis may be associated with the up -regulated mRNA of retinoic acid receptor α.