摘要
[Objective] The aim was to study on the chlorella resistance to dehydration conditions and effect of different cryoprotecants on drying of chlorella cells.[Method]The protection effect of seven kinds of cryoprotecants on chlorella cells was analyzed.[Result]The protection effect of trehalose on chlorella cells was the best,and the survival rate of chlorella cells had improved by 50% with the applied trehalose concentration of 5%.[Conclusion]The result in this study had important guidance effect on the mutation breeding of chlorella.
[目的]研究小球藻的风干致死及干燥保护方法。[方法]比较7种保护剂对处于对数生长期的小球藻细胞的保护作用。[结果]海藻糖对藻细胞的保护效果最好,当海藻糖用量为5%时,藻细胞存活率提高了50%。[结论]该研究结果对淡水微藻的诱变育种具有重要指导意义。
基金
Support by a Grant from the Major State Basic Research Development Program of China(973 Program,2009CB724702)
Science Foundation of Nanjing Industry University(39708010)~~