Development of a Multivalent Inactivated Vaccine against Pathogenic Escherichia coli Infection in Forest Musk Deer

林麝大肠杆菌病三价铝胶灭活疫苗的研制及其抗体水平测定(英文)

A multivalent inactivated Escherichia coli vaccine for forest musk deer by using serotypes O4,O26,and O139 with Al（OH）3 adjuvant was prepared.The vaccine did not cause any adverse reactions in forest musk deer.The immunogenic effects of the vaccine were experimentally investigated in pregnant and young forest musk deer.The serum antibody titers of pregnant and young forest musk deer were determined by performing the micro-agglutination test.The serum antibody titers of pregnant forest musk deer were more stable from 35th to 68th d after the third vaccination,and the serum antibody titers of four pregnant forest musk deer were maintained 25,25,25,and 24 on 68th d after the third vaccination.Young forest musk deer showed serum antibody titers which were obtained due to nursing.Young forest musk deer were administered the first intramuscular vaccine injection at an age of approximately 60 days due to a fall in maternal antibody titers.The serum antibody titers of young forest musk deer were higher after the third vaccination and maintained at approximately the same level until they were 137 days old.The maternal antibodies and the antibodies produced by young forest musk deer could be helpful for protecting the young musk deer from the infections of pathogenic Escherichia coli strains（serotypes O4,O26,and O139）for 137 days after birth（during the nursing period and the period when the forest musk deer were susceptible to diseases）.

采用林麝致病性大肠杆菌血清型菌株O4,O26和O139为抗原,经甲醛灭活,以铝胶为佐剂,制成林麝大肠杆菌病三价铝胶灭活疫苗,含菌量为20×108/ml。疫苗成品纯粹检验、安全性检验均达到相关要求。将疫苗分别肌肉注射怀孕母麝及其所产仔麝,采用首免后7天进行二免及其二免后14天进行三免的免疫方法,并用微量血凝试验检测母麝和仔麝的血清抗体水平。结果表明:怀孕林麝产后35天至产后68天的血清抗体水平较稳定,怀孕林麝产后68天的血清抗体仍可维持到平均24.75;仔麝可获得母源抗体,但母源抗体在出生后60天消退,因此仔麝首免时间确定为出生后60日龄,仔麝三免后可获得一定的抗体水平,可使仔麝对林麝致病性大肠杆菌有一定的保护力。该疫苗的试制为致病性大肠杆菌性化脓病和肺炎的预防奠定了一定的基础。