蠲痹强骨颗粒质量标准研究

Study on quality standards of Juanbiqianggu particles

目的建立蠲痹强骨颗粒的质量标准。方法采用薄层色谱法对处方中的穿山龙、枸杞子、淫羊藿等药材进行鉴别;采用高效液相色谱法测定淫羊藿苷的含量：色谱柱为Hypersil BDS C18柱（250mm×4.60mm,5μm）;流动相为乙腈-水30∶70）,流速1.0mL.min-1;检测波长为270nm,进样量为10μL。结果穿山龙、枸杞子、淫羊藿的TLC斑点清晰、分离度良好。HPLC法测定淫羊藿苷含量,在0.1~2.0μg范围内线性关系良好,平均回收率为101.85%,RSD为1.71%。结论所建质量标准简便可行、重复性好,可用于蠲痹强骨颗粒的质量控制。

OBJECTIVE To establish the quality standard of Juanbiqianggu Particles.METHODS Rhizoma dioscoreae nipponicae,Lycium barbarum L.and Epimedium were identified with TLC.The content of icariin was determined by HPLC at 270nm on a Hypersil BDS C18 column（250mm×4.60mm,5um）with acetonitrilewater（30：70） as mobile phase and flow rate of 1.0mL·min-1.RESULTS The Rhizoma dioscoreae nipponicae,Lycium barbarum L.and Epimedium could be identified by TLC.The linear range of calibration curve was 0.1～2.0μg,the average recovery was 101.85% with RSD of 1.71%.CONCLUSION The method was simple,accurate,reliable,and can be used as method for quality control of the production.