顺铂脂质体腹腔注射对S180荷瘤小鼠的治疗作用

The Effects of Cisplatin Liposomes on S180 Tumor Bearing Mice

目的探讨顺铂脂质体腹腔注射对S180荷瘤小鼠的抗肿瘤作用。方法采用昆明种小鼠腹腔内注射S180细胞形成腹水瘤模型,40只小鼠肿瘤种植24 h后随机分为4组,分别为生理盐水组、空白脂质体组、顺铂注射液组、顺铂脂质体组,分别给予生理盐水、空白脂质体、顺铂注射液和顺铂脂质体腹腔注射,共给药4次,给药时间间隔72 h。结果肿瘤种植14 d后,生理盐水组、空白脂质体组、顺铂注射液组小鼠形成大量腹水,腹膜广泛转移;顺铂脂质体组仅形成少量腹水,腹膜未见转移。生理盐水组、空白脂质体组、顺铂注射液组小鼠解剖后均发现肠系膜上多发粟粒状结节改变;顺铂脂质体组小鼠解剖后发现肠系膜外观均较光整,未发现肠系膜上粟粒状结节改变。与生理盐水组、空白脂质体组相比,顺铂注射液组和顺铂脂质体组小鼠的生存期明显延长(P<0.05),且顺铂脂质体组明显长于顺铂注射液组(P<0.05)。结论顺铂脂质体腹腔注射可以有效抑制S180荷瘤小鼠腹水形成和腹腔转移。

Objective To investigate the antitumor effects of cisplatin liposomes on S180 tumor bearing mice. Methods Forty Kunming mice bearing ascites tumor xenografts were randomly divided into four groups ： the physiological saline group, the blank liposomes group, the cispla- tin injection group and the cisplatin liposomes group. After 24 hours of implantation, the mice in the four group received respectively physio- logical saline,blank liposomes, cisplatin injection and cisplatin liposomes for 4 times, the dosing interval was 72 hours. Results The mice had resembly much ascites and peritoneal metastases in the physiological saline group, the blank liposomes group and the cisplatin injection group ; but had a small amount of ascites and no peritoneal metastases in the cisplatin ]iposomes group. The mesenteric multiple military nod- ules were observed in the physiological saline group, the blank liposomes group, the cisplatin injection group; but no mesenteric muhiple mili- tary nodules in the cisplatin liposomes group. Compared with the physiological saline group and the blank liposomes group, the survival time was longer in the cisplatin injection group and the cisplatin liposomes group（ P 〈 0.05 ） ; the survival time in the cisplatin liposomes group was longer than that of the cisplatin injection group（P 〈 0. 05）. Conclusion Intraperitoneal injection of cisplatin liposomes can effectively inhibit the formation of ascites and peritoneal metastases in the S180 tumor bearing mice.