摘要
采用电喷雾-四极杆-飞行时间质谱(ESI-Q-TOF MS)在负离子检测方式下,对仿刺参中4种皂苷类成分的裂解途径进行研究。通过ESI-MS产生的[M-H]-获得相应化合物的相对分子质量信息,对[M-H]-进行碰撞诱导解离(CID)获得相应化合物的裂解途径信息。研究发现,4种皂苷化合物的ESI-MS/MS裂解规律相同,首先断裂苷元上的18(20)内酯键,然后连续丢失糖链的末端单糖,最后生成苷元的特征碎片离子m/z423,这些特征有助于皂苷类化合物的结构解析。
The fragmentation pathways of four triterpene glycosides from Apostichopus japonicus Selenka were investigated in negative ion mode by ESI-Q-TOF MS.The -ions were observed by ESI-MS,from which the molecular weights were obtained.The collision induced dissociation(CID) data of the -ions provide fragmentation pathways of the triterpene glycosides.Results show that four compounds have the same fragmentation pathway.The cleavage is initiated by a loss of 18(20)-lactone residue from the aglycon,then consecutively loses monosaccharide residue of sugar chain,and finally yields a signature ion peak at m/z 423.These characteristic fragmentations will be helpful in identifying the structures of triterpene glycosides.
出处
《质谱学报》
EI
CAS
CSCD
2011年第2期77-81,共5页
Journal of Chinese Mass Spectrometry Society
基金
国家自然科学基金(31000795)
海洋公益性行业科研专项(201105029)
国际科技合作项目(2010DFA31330)资助
关键词
仿刺参
皂苷
质谱
裂解规律
Apostichopus japonicus Selenka
triterpene glycosides
mass spectrometry
fragmentation pathways