高效液相色谱法测定纳豆及纳豆胶囊中的大豆异黄酮含量

Determination of Soybean Isoflavones in Natto and Natto Capsule by HPLC

建立了纳豆及纳豆胶囊中大豆异黄酮的高效液相色谱分析方法,该方法重复性及样品稳定性良好.实验对原料黄豆、纳豆和纳豆胶囊样品采用石油醚索氏脱脂后,对固体样品进行乙醇回流提取,分析了4种大豆异黄酮组分,即大豆苷、染料木苷、大豆素和染料木素.结果表明,原料黄豆总异黄酮质量比为1 260 mg/kg,纳豆比原料黄豆总异黄酮含量明显高约30%,纳豆胶囊比原料黄豆总异黄酮含量高约11%.

A high performance liquid chromatographic method was developed for the determination of soybean isoflavones in Natto and Natto Capsule,and the method showed good repeatability and sample stability.Soybean isoflavone were extracted from defatted soybean,Natto and Natto capsule by heating circumfluence method.Four kinds of soybean isoflavone including daidzin,genistin,daidzein and genistein were determined.The results showed that the soybean isoflavones in Natto were 1 260 mg/kg,which were 30% higher than that in soybean,and the soybean isoflavones in Natto capsule were 11% higher than that in soybean.