双(7)他克林对大鼠海马缺血性损伤的保护作用

Protective Effect of Bis (7) -tacrine to Hippocampal Neural Leision in Cerebral Hypoperfusion in Rat

目的:研究双(7)他克林对大鼠缺血性脑损伤中海马神经元损伤的作用.方法:永久性结扎大鼠双侧颈总动脉建立老年性痴呆模型,手术动物随机分成2组,手术7 d后分别给以双(7)他克林和生理盐水灌胃,给药7d后灌注固定处死动物,取海马组织,常规脱水包埋,连续切片.分别进行常规HE染色、凋亡检测、PCNA及GFAP免疫组织化学染色.在海马CA1区、CA2区、CA3区和齿状回区取凋亡细胞及PCNA、GFAP阳性细胞进行连续图片采集后计数.结果:经双(7)他克林治疗7d后,大鼠海马区颗粒细胞层细胞凋亡现象有不同程度的降低,CA1区、CA3区和齿状回区更为明显(P值:0.03,0.00,0.03);PCNA阳性细胞出现明显增多(P值:0.00,0.03,0.00,0.00);颗粒细胞层细胞出现不同程度增多,胶质细胞的密度增加,星形细胞的胞质突起增多,以CA1区最为明显(P值:0.04).结论:双(7)他克林在大鼠海马早期缺血性损伤时不仅具有抗凋亡的作用,还具有激活大脑自身修复机制的双重效应,还可能通过促进星形胶质细胞的增生以及形态结构的改变,减轻神经细胞的凋亡而实现对海马神经元的保护作用.

Objective：To explore effects of Bis（7）-tacrine to hippocampal neural leision in cerebral hypoperfusion in rat.Methods：Permanent bilateral occlusion of the common carotid arteries（2VO） in rats has been established.11 SD rats were randomly divided into control group and B7T group.Investigated cell proliferation and apoptosis of hippocampal CA1,CA2,CA3 and gyrus dentatus by HE,immunohistochemistry and immunofluorescence stain in 2VO rats.Results：7 days after treatment with Bis（7）-tacrine in rats,results indicated more PCNA-labeled cells,more positive cells of GFAP and less apoptosis cells in hippocampal CA1,CA2,CA3 and gyrus dentatus region than those of the control group.Conclusion：The results indicate administration of B7T can inhibit ischemia-induced cell apoptosis and promote cell proliferation in acute cerebral ischemia rats.