摘要
苏云金杆菌XPZ-9菌株是从土壤中分离的对鳞翅目幼虫有特异杀虫活性的新菌株。光学显微镜下观察到该菌株可产生菱形和圆形伴胞晶体,SDS-PAGE检测表达的主要蛋白条带分子量约为70 kDa。PCR-RFLP分析结果表明该菌株含有新基因cry1Ah。生物活性测定表明该菌株对鳞翅目害虫棉铃虫、小菜蛾、亚洲玉米螟和美国白蛾的二龄幼虫都具有较强的杀虫活性。
A high toxicity Bacillus thuringiensis（Bt）strain XPZ-9 against Lepidoptera pests was isolated and studied further.The strain formed the bipyramidal crystal and spherical crystal observed by optical microscope.SDS-PAGE analysis revealed molecular weight of insecticidal crystal protein in this strain was 70 kDa.By using of PCR-RFLP identification method,new cry1Ah gene was found in this strain.The bioassay results indicated Bt XPZ-9 toxin protein had high insecticidal activity against several Lepidoptera pests,such as 2 instar larvae of Helicoverpa armigera,Plutella xylostella,Ostrinia furnacalis,Hyphantria cunea.
出处
《华北农学报》
CSCD
北大核心
2011年第1期54-57,共4页
Acta Agriculturae Boreali-Sinica
基金
国家高技术研究发展专项经费资助
关键词
苏云金杆菌
小菜蛾
cry1Ah
美国白蛾
Bacillus thuringiensis
Plutella xylostella
cry1Ah
Hyphantria cunea
