高密度养殖下革胡子鲶生长激素mRNA表达的半定量分析

Semi-quantitative Analysis of Growth Hormone(GH)mRNA Expression of Clarias gariepinus in High Density Pond Culture

以β-肌动蛋白(β-actin)mRNA的表达量作为内标,应用半定量RT-PCR法,对高密度养殖条件下不同月龄革胡子鲶垂体生长激素(Growth hormone,GH)mRNA的相对表达量进行分析。结果显示:3.5,5,6.5月龄试验鱼的GH mRNA的相对表达量分别为3.612～5.965,2.929～4.556和1.360～1.821;平均相对表达量分别为4.346±0.656 0,3.649±0.568 4和1.575±0.135 5。上述数据经方差分析得出,革胡子鲶任意两个不同月龄之间GH mRNA相对表达量的差异达到1%的显著水平。Duncan氏多重比较结果显示,3.5,5月龄试验鱼垂体GH mRNA的平均相对表达量差异不显著,但均极显著高于6.5月龄。

Using β-actin mRNA expression quantity as an internal reference,the differences of growth hormone（GH）mRNA expression in pituitary of Clarias gariepinus in high density pond culture at different months old were analyzed with the employment of semi-quantitative RT-PCR method.The relative expression quantity of GH mRNA of individuals at 3.5,5 and 6.5 months old were 3.612-5.965,2.929-4.556 and 1.360-1.821,respectively,while the mean relative expressions were 4.346±0.656 0,3.649±0.568 4 and 1.575±0.135 5 separately for 3.5,5 and 6.5 months old.The variance analysis indicated that the significant differences reached 1%in the relative expression level of the GH mRNA between any two months old.The Duncan′s multiple comparison showed that the differences of GH mRNA expression between that at 3.5 and 6.5 months old as well as that at 5 and 6.5 months old were extremely significant,but there was no difference of GH mRNA expression between that at 3.5 and 5 months old.