与黄瓜抗炭疽病相关基因连锁的基因片段及SCAR标记

Resistance-related Gene Fragments and SCAR Marker for Identification of Cucumber Anthracnose Resistance

为建立黄瓜抗炭疽病分子标记辅助选择技术体系,以黄瓜抗病亲本66和感病亲本A18以及它们的F1、F2、BC1群体为试材,对黄瓜抗炭疽病的遗传规律进行了分析,结果表明,其抗性是由一对单隐性基因控制的,感病相对抗病为不完全显性。将炭疽病抗性相关基因的一个共显性AFLP标记进行了测序,根据序列特点设计了特异的SCAR引物SCEM178/172,并成功地转换成了简单实用的共显性SCAR标记,结果显示,该标记可以作为黄瓜抗炭疽病辅助选择的标记,且具有迅速、简便、成本低、不受环境条件限制,扩增条带清晰,无杂带和拖尾现象的特点,适合用于大量样本分析。最后,利用引物SCEM178/172对288份材料进行抗病性检测,结果表明,有138份抗病材料,这为进行抗炭疽病黄瓜新品种的选育奠定了基础。

In order to establish molecular marker-assisted selection system of cucumber anthracnose,with F1,F2 and BC1 population between a resistant parent（66）and susceptible parent（A18）,we found that the resistance to anthracnose was controlled by a pair of recessive gene,and the susceptible was in the mode of incomplete dominant inheritance.A co-dominant AFLP marker was successfully converted into a simple,applied and co-dominant SCAR marker.Special marker SCEM178/172 was designed according to the sequence information of AFLP fragments,the marker could be useful in marker-assisted selection in cucumber breeding.The acquired SCAR marker had many advantages including fast,accurate,low cost,and free from an environmental influence,application of the acquired marker will enhance cucumber anthracnose disease resistant germplasm evaluation.138 resistant germplasm were obtained from total 288.