急性拥挤胁迫对半滑舌鳎血液指标的影响

Effect of Acute Crowding Stress on Plasma Index of Half-smooth Tongue-sole

为了解高密度养殖对鱼体的影响规律及作用机制,研究了短时间(3 h)急性拥挤(14 kg/m2)胁迫后,半滑舌鳎部分血液指标的变化。结果显示,胁迫后CAT(过氧化氢酶)活力下降并在12 h降到最低(P<0.05),24 h恢复到胁迫前水平(3 kg/m2);胁迫后GSH(还原型谷胱甘肽)显著下降,24 h降到最低,之后逐渐升高但仍与胁迫前差异显著(P<0.05);MDA(丙二醛)含量呈波动性变化,胁迫后4 h升至最高,并与胁迫前差异显著(P<0.05),随后下降,48 h后又升高;胁迫后LZM(溶菌酶)活力显著地升高,4 h升至最高(P<0.05),之后逐渐下降,48 h恢复到胁迫前水平;补体C3、C4含量胁迫后明显升高,各时间段内均显著高于胁迫前水平(P<0.05);Alb/Glb(白蛋白/球蛋白)胁迫后逐渐升高,12 h与胁迫前差异显著(P<0.05),24 h显著降低(P<0.05),并持续至192 h;胁迫后4～12 h CHO(总胆固醇)含量显著高于胁迫前(P<0.05),之后恢复到胁迫前水平;胁迫后Glu(血糖)含量升高,4～192 h均显著高于胁迫前(P<0.05);TP(总蛋白)和Glb均在胁迫后24 h显著高于胁迫前并维持较高水平(P<0.05);胁迫后4 hALT(丙氨酸氨基转移酶)活力显著高于胁迫前(P<0.05),之后逐渐下降至胁迫前水平;胁迫后48 h AST(天门冬氨基转移酶)活力显著高于胁迫前(P<0.05),之后下降至胁迫前水平;胁迫后SOD(超氧化物歧化酶)活力、Alb和TG(甘油三酯)含量变化不大(P>0.05)。测试结果表明,短时间高密度胁迫对半滑舌鳎的生理机能不会产生持久影响,却可以提高鱼体免疫力。

The short（3 h）acute crowding（14 kg/m2）stress changes in some blood indices of half-smooth tongue-sole was studied to understand the high-density fish culture on the influence and mechanism of action.Results showed that CAT activity decreased and reached to trough at 12 h（P〈0.05）and restored to the level before stress at 24 h;GSH content decreased significantly and reached the lowest point at 24 h after stress,increased afterwards but also showed significant differences with that before stress（P〈0.05）;There was fluctuation in MDA content,it reached to peak after stress 4 h and showed significant differences with that before stress;then decreased and increased after 48 h;LZM vitality rose markedly after stress,reached to highest after stress 4 h,then reduced gradually,restored to the level before stress at 48 h;C3 and C4 content elevated significantly after stress,and the level was significant higher than that before stress（P〈0.05）;A/G increased gradually after stress,and there was significant differences with the level of 12 h and before stress（P〈0.05）,then reduced significantly at 24 h（P〈0.05）and continued to 192 h;CHO content was significant higher than that before stress after stress 4-12 h（P〈0.05）,and recovered to the level before stress;Glu content elevated after stress,and the level of 4-192 h was significantly higher than that before stress（P〈0.05）;Total protein and globulin of stress 24 h were significant higher than that before stress and maintained at high level（P〈0.05）;after stress 4 h,ALT activity was marked higher than that before stress（P〈0.05）,then reduced to the level before stress;After stress 48 h,AST vitality was significant higher than that before stress,and reduced to the level before stress afterwards;There was no changes in TG,SOD and ALB after stress（P〈0.05）.The results showed that short high-density stress on physiological functions of half-smooth tongue-sole would not have a lasting impact and it could improve immunity of half-smooth tongue-sole.