慢性支气管炎肺气虚证大鼠转化生长因子-β_1的变化及其意义

Changes of TGF-β_1 and its significance of chronic bronchitis rats with syndrome of insufficiency of lung-QI

目的探讨慢性支气管炎肺气虚证大鼠血清及肺组织中抗炎细胞因子转化生长因子β1(TGF-β1)的变化及其意义。方法将40只SD大鼠随机分为空白对照组(对照组)和慢性支气管炎肺气虚证组(模型组),各20只,雌雄各半。模型组大鼠采用改良烟熏法,复制慢性支气管炎肺气虚证模型,检测血清及肺组织中TGF-β1的含量,探讨慢性支气管炎肺气虚证状态下机体炎症因子的变化特点。结果模型组大鼠血清、肺组织中TGF-β1含量均较对照组明显降低(P<0.01)。结论机体TGF-β1水平的降低,是慢性支气管炎发病的重要使动因素,其水平变化可以作为临床肺气虚证微观辨证诊断的重要参考指标。

Objective To investigate the transforming growth factor β1（TGF-β1） changes in serum and lung tissue of chronic bronchitis rats with syndrome of insufficiency of lung-QI.Methods With modified method of smoking to duplicate models of chronic bronchitis rats with syndrome of insufficiency of lung-QI,40 rats were randomly divided into two groups,such as the normal group and the model group,20 rats was in each group.To detect the TGF-β1 contents in serum and lung tissue,for investigating the changes characteristic of inflammatory factor in status of chronic bronchitis rats with syndrome of insufficiency of lung-QI.Results The TGF-β1 contents had decreased obviously in model group compared with that of control group（P0.01）.Conclusion The TGF-β1 changes is the preliminary factor of chronic bronchitis,and the TGF-β1 level could be acted as the important reference index of syndrome of insufficiency of lung-QI.