苯及其代谢产物在小鼠骨髓中形成DNA加合物的实验研究

D N Aadduct formation in the bone m arrow of mice treated with benzene and some metabolites

目的 观察苯及其主要代谢产物苯酚、氢醌、苯醌及苯酚和氢醌联合染毒在小鼠骨髓中形成的 Ｄ Ｎ Ａ 加合物及其髓性毒性。方法 改进的３２ Ｐ 后标记方法。结果 苯及其主要代谢产物均能在小鼠骨髓细胞中形成至少１ 种 Ｄ Ｎ Ａ 加合物，相对加合物水平从０ ．８５ ×１０ － ８ ～６ ．７８ ×１０ － ８ 不等。骨髓细胞计数结果表明：单独染苯酚或氢醌，对小鼠骨髓细胞均没有抑制作用，但二者联合染毒对小鼠却表现出增强的髓性毒作用。结论 苯及其主要代谢产物均能在小鼠骨髓细胞中形成 Ｄ Ｎ Ａ 加合物，苯的代谢产物形成的 Ｄ Ｎ Ａ 加合物与髓性毒性有关。

Objective To investigate D N Aadduct formation induced by benzene and some metabolitesin the bone marro w and the m yelotoxicity . Methods Female Kun ming mice were treated intraperioneallywith benzene and some metabolites twice daily ,and nuclease P1 enhanced 32 P postlabelling assay was used tostudy the effect . Results No adduct was detected in the bone marrow of control group . At least one major D N A adduct was detected in the bone marrow of mice treated with benzene ,phenol,hydroquinone ,benzo quinone respectively and coadministration of phenol and hydroquinone . The relative adduct levels ranged from0 .85 ×10 - 8 ～6 .78 ×10 - 8 . Ad ministration of phenol(75 m g/kg) or hydroquinone (75 m g/kg) twice dailyfor 12 days did not result in a suppression of bone m arrow cellularity respectively . However ,the conco mitantad ministration of phenol(75 m g/kg) and hydroquinone(50 m g/kg) twice a day for 12 days produced a signif icant decrease in bone m arrow cellularity . Conclusion Benzene and some metabolites showed myelotoxicityby forming adductin the bone marro w .