葡多酚对小鼠DNA与染色体放射性损伤的防护作用

Study on the protective effect of grape polyphenol on D N Aand chrom oso me dam age induced by irradiation inmice

目的 探讨葡多酚对辐照诱发的 Ｄ Ｎ Ａ 与染色体损伤的防护作用。方法 将小鼠经口灌胃给予不同剂量葡多酚，采用６０ Ｃｏ γ射线全身照射１ 次，照射后第２ 天检测各组动物的脾细胞 Ｄ Ｎ Ａ 损伤、骨髓嗜多染红细胞微核率和肝脏丙二醛（ Ｍ Ｄ Ａ） 含量３ 项指标。结果 同阳性对照组比较，各试验组的 Ｄ Ｎ Ａ 损伤程度、微核率和 Ｍ Ｄ Ａ 水平均有不同程度降低。经ｔ （ 或 χ２） 检验，差异均有显著性意义（ Ｐ＜ ０ ．０５） 。结论 葡多酚对放射线照射引发的 Ｄ Ｎ Ａ 和染色体损伤有良好的防护作用。

Objective To study the protective effect of grape polyphenol( G P P) on D N Aand chro mo som e damage induced by irradiation . Method Three indices including spleen cell D N A dam age ,bone m arrowon P C Es and liver M D Acontent were examined in the mice given different dose of G P Pby mouth were radiat ed by 60 Co γ ray once . Results Com pared with that in positive control group ,the degrees of D N A dam age ,rate of micronucleus and M D A content of mice reduced differently in the protected groups . All the differencesabove are signifcantly in statistic( P < 0 .05) . Conclusion The present experiment proves that G P P hasdominant protective effect on D N A and chro moso me dam age induced by irradiation .