维生素D缺乏动物模型的建立

To establish the animal model of vitamin D deficiency

目的 采用BALB/c小鼠建立维生素D（VD）缺乏模型并进行评价.方法 将BALB/c小鼠分成正常组、VD＋组及VD-组,测4～10、12周小鼠体质量,并观察其运动习性,8、12周期间饮食消耗;12周时测血浆VD、清蛋白水平;将VD缺乏老鼠10只分为组Ⅰ继续遮光加正常饮食,组Ⅱ正常环境加正常饮食,6周后测VD水平.结果 正常组与VD＋组体质量比较差异无统计学意义（P〉0.05）;VD＋组与VD-组8周时日平均消耗饲料,有差异,12周时无差异;正常组、VD＋组、VD-组血浆清蛋白分别为（33.2±1.04）、（32.4±0.53）、（32.8±0.67）g/L,差异无统计学意义;正常组与VD＋组血浆VD水平无显著差异,VD-组血浆VD水平下降到正常的20%以下;6周后,组Ⅰ、组Ⅱ VD水平分别为（18.46±1.53）、（58.46±5.53）nmol/L（P＜0.001）.结论 通过控制维生素来源建立维生素D缺乏BALB/c小鼠动物模型是可行的.

Objective To establish and evaluate the animal model of vitamin d deficiency using BALB/c mice. Methods Put BALB/c mice into normal group, VD＋ group andVD-group, measured the weight of the three groups of 4--10,12th week; ob served the mouse activity;Recorded food consumption during 8 and 12th week;Assay for serum albumin and 25-hydroxyvitamin D level after 12 weeks. Then 10 mouse of vitamin D deficiency were divided into two groups with normal diet for six weeks,but one group as beginning the other one as normal group,then measured the serum level of 25-hydroxyvitamin D. Results Normal group and VD@ group had no statistical differences in body weight（P〉0.05） ;At 8th week ,the average consumption of synthesis food in VD＋ group was （6.72±0.89）g/d,VDT- group was （5.82±1.34）g/d;At 12th week times VD＋ group was （7.05±0.72）g/d, VD-group was （6.67±0.84）g/d, there was a little difference at 8th week but no statistical differences at 12th weeks;The plasma albumin of normal group, VD＋ group, VD-group were （33.2 ± 1.04）, （32.4±0.53）, （ 32.8 ±0.67 ） g/L, respect ively. Two of the three groups and between the two groups were no statistically differences;Serum 25-hydroxyvitamin D had no significant differences between normal group and VD＋ group, but significant differences in VD-- group with decreased into normal 20%The serum 25- hydroxyvitamin D of two deficiency groups after six months were （18.46±1.53） and （58.46±5.53）nmol/L（P〈0. 001）. Conclusion The establishment of vitamin deficiency BALB/c mice animal model by controlling the sources of vitaminD is feasible.