禽呼肠孤病毒ZJS株种毒的纯净性鉴定

Purity and Identity Test for Reovirus Seeds(Strain ZJS)

将禽呼肠孤病毒ZJS株细胞毒(弱毒)在鸡胚成纤维细胞(CEF)上连续传代,对其C1、C2、C3代种毒进行了无菌和支原体污染检验,对C5、C8和C10代种毒进行了外源病毒污染检验,对C3、C8和C10代种毒还进行了特异性鉴定,以确定禽呼肠孤病毒ZJS株细胞毒(弱毒)作为种毒的可行性。结果显示,禽呼肠孤病毒ZJS株细胞毒(弱毒)C1、C2、C3代种毒未污染细菌、霉菌和支原体,C5、C8和C10代种毒未污染外源病毒,C3、C8和C10代种毒能被特异性阳性血清中和,表明禽呼肠孤病毒ZJS株细胞毒(弱毒)纯净性和特异性鉴定结果均符合规定。

Reovirus seeds（strain ZJS） were continuously passaged on primary CEF.Sterility and mycoplasmas test were done on passage 1,2 and 3.Extraneous viruses contamination test were taken on passage 5,8 and 10.No bacteria,fungi and mycoplasmas can be found for passage 1,2 and 3.No extraneous viruses can be detected for passage 5,8 and 10,and the seed viruses were neutralized with antiserum of reovirus in the identity test for passage 3,8 and 10.The results showed that reovirus seeds（strain ZJS） complied with the purity and identity requirements for seed virus.