瑞舒伐他汀对兔动脉粥样硬化模型中IL-8的干预研究

Effect of rosuvastatin on IL-8 expression of atherosclerotic rabbits

目的观察瑞舒伐他汀对兔动脉粥样硬化模型中白细胞介素8(IL-8)水平及其表达的影响。方法 30只新西兰大白兔随机分为对照组,实验组,治疗组,每组10只。对照组以标准基础饲料喂养,实验组和治疗组以高脂饲料喂养4周后,行颈动脉内膜球囊损伤术,分别继续予高脂饲料,及高脂饲料加瑞舒伐他汀喂养6周。于4周末及10周末抽血测定血脂及IL-8。于10周末将兔处死时,取颈动脉标本行病理切片,免疫组织化学方法检测IL-8的表达。结果 4周末与对照组相比,实验组及治疗组血脂及IL-8水平均明显升高(P<0.05)。10周末治疗组血脂及IL-8水平均明显低于实验组(P<0.05);正常对照组颈动脉标本未见动脉硬化病变及IL-8表达;治疗组中动脉内膜面积(IA)、内膜/中膜面积比(I/M)、管腔狭窄度(LSD)、动脉粥样硬化斑块中IL-8的表达明显低于实验组(P<0.05)。结论瑞舒伐他汀可降低血脂及IL-8的水平并减少其表达,具有抗炎、抑制动脉粥样硬化进展的作用。

Objective To observe the influence of rosuvastatin on IL-8 and its expression in rabbit atherosclerosis model.Methods 30 New Zealand rabbits were randomly divided into three groups： control group（n=10）,experiment group（n=10） and treatment group（n=10）.Animals in control group received normal diet,the other two groups received arterial intimal injury of carotid with balloon after four weeks experiment feeding,then treated with experiment,experiment and rosuvastatin for six weeks,respectively.3 ml blood was drawn from the ear vein of all the rabbits after the carotid arterial intimal injury（at the end of the fourth week） and before sacrifice（at the end of the tenth week）.Lipid and IL-8 levels were measured.At the end of the experiment,all rabbits were sacrificed,the carotid arteries were isolated and paraffin-embedded slices and immunohistochermical staining of IL-8 were performed.Results Compared with control group,Lipid and IL-8 levels in treatment group and experiment group were significantly increased at the end of 4 weeks（P0.05）,and lipid and IL-8 levels in treatment group were significantly lower than experiment group at the end of 10 weeks（P0.05）;There were no arteriosclerosis lesions and IL-8 expression in the carotid arteries of control group;compared with experiment group,intima area（IA）,ratio of intima area to membrane area（I/M）,luminal stenosis degrees（LSD） and the expression of IL-8 on atherosclerotic plaque were significantly decreased in treatment group（P0.05）.Conclusion Rosuvastatin can reduce IL-8 level and decrease expression of IL-8 in atherosclerosis plaques.It demonstrates that rosuvastatin may inhibit the progression of atherosclerosis by reducing the inflammatory action.