摘要
目的 探讨淋巴细胞因子激活的杀伤细胞/ 活化自然杀伤细胞(LAK 细胞/ 活化NK 细胞)与造血的关系及其对同基因骨髓移植小鼠造血重建的影响。方法 ①将同基因LAK 细胞/ 活化NK 细胞与骨髓细胞一起置于体外半固体琼脂培养体系中,观察其对CFUGM 形成的影响。②在小鼠同基因骨髓移植中, 联合注射同基因LAK 细胞/ 活化NK 细胞, 观察其对移植后小鼠造血重建的影响。结果①当骨髓细胞置于有外源性集落刺激因子(CSF) 的适宜培养体系中,同基因LAK 细胞/ 活化NK 细胞抑制CFUGM 形成;而当骨髓细胞置于无CSF 不良培养体系中,同基因LAK 细胞/ 活化NK 细胞促进CFUGM 形成。②在小鼠同基因骨髓移植中,联合注射同基因LAK 细胞/ 活化NK 细胞,能显著促进CFUS 和CFUGM 增殖和显著升高白细胞和血小板数量。结论 ①同基因LAK 细胞/ 活化NK 细胞对CFUGM 形成有双向调节作用。②在小鼠同基因骨髓移植中,联合注射同基因LAK 细胞/ 活化NK 细胞,能促进移植后小鼠造血重建。
Objective To study the relationship between LAK/activated NK cells and hematopoiesis as well as the effects of LAK/activated NK cells on hematopoietic reconstitution after syngeneic bone marrow transplantation(BMT) in mice. Methods ①LAK/activated NK cells were cocultured with bone marrow cells(BMC) in semi solid agar medium to determine their effects on CFU GM formation. ②Syngeneic LAK/activated NK cells were transferred with BALB/c BMCs into lethally irradiated syngeneic recipients to determine their effects on hematopoietic reconstitution. Results When BMC were cultured under optimal growth conditions in the presence of exogenous hematopoietic growth factors, the effects of LAK/activated NK cells were mainly inhibitory. However, when BMCs were cultured under growth limiting conditions without exogenous hematopoietic growth factors, the LAK/activated NK cells could support CFU GM formation. Syngeneic LAK/activated NK cells significantly increased CFU S and CFU GM numbers after syngeneic BMT,and also significantly improved platelet and white blood cell count. Conclusions LAK/activated NK cells could be bidirectionally regulate CFU GM formation in vitro and promote hematopoietic reconstitution after syngeneic BMT in mice.
出处
《中华血液学杂志》
CAS
CSCD
北大核心
1999年第8期405-408,共4页
Chinese Journal of Hematology
基金
1997 年山东省自然科学基金
关键词
LAK细胞
NK细胞
骨髓移植
造血功能
LAK cells NK cells Hematopoiesis Bone marrow transplantation