LAK细胞和活化NK细胞对小鼠骨髓造血功能影响的实验研究

Experimental study of LAK and activated NK cells affecting hematopoiesis in mice

目的 探讨淋巴细胞因子激活的杀伤细胞／ 活化自然杀伤细胞（ＬＡＫ 细胞／ 活化ＮＫ 细胞）与造血的关系及其对同基因骨髓移植小鼠造血重建的影响。方法 ①将同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞与骨髓细胞一起置于体外半固体琼脂培养体系中，观察其对ＣＦＵＧＭ 形成的影响。②在小鼠同基因骨髓移植中， 联合注射同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞， 观察其对移植后小鼠造血重建的影响。结果①当骨髓细胞置于有外源性集落刺激因子（ＣＳＦ） 的适宜培养体系中，同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞抑制ＣＦＵＧＭ 形成；而当骨髓细胞置于无ＣＳＦ 不良培养体系中，同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞促进ＣＦＵＧＭ 形成。②在小鼠同基因骨髓移植中，联合注射同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞，能显著促进ＣＦＵＳ 和ＣＦＵＧＭ 增殖和显著升高白细胞和血小板数量。结论 ①同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞对ＣＦＵＧＭ 形成有双向调节作用。②在小鼠同基因骨髓移植中，联合注射同基因ＬＡＫ 细胞／ 活化ＮＫ 细胞，能促进移植后小鼠造血重建。

Objective To study the relationship between LAK/activated NK cells and hematopoiesis as well as the effects of LAK/activated NK cells on hematopoietic reconstitution after syngeneic bone marrow transplantation(BMT) in mice. Methods ①LAK/activated NK cells were cocultured with bone marrow cells(BMC) in semi solid agar medium to determine their effects on CFU GM formation. ②Syngeneic LAK/activated NK cells were transferred with BALB/c BMCs into lethally irradiated syngeneic recipients to determine their effects on hematopoietic reconstitution. Results When BMC were cultured under optimal growth conditions in the presence of exogenous hematopoietic growth factors, the effects of LAK/activated NK cells were mainly inhibitory. However, when BMCs were cultured under growth limiting conditions without exogenous hematopoietic growth factors, the LAK/activated NK cells could support CFU GM formation. Syngeneic LAK/activated NK cells significantly increased CFU S and CFU GM numbers after syngeneic BMT,and also significantly improved platelet and white blood cell count. Conclusions LAK/activated NK cells could be bidirectionally regulate CFU GM formation in vitro and promote hematopoietic reconstitution after syngeneic BMT in mice.