肺癌组织中血管内皮生长因子表达的定量研究

Quantitative study of vascular endothelial growth factor expression in primary lung cancer

目的 研究肺癌组织中血管内皮生长因子（ Ｖ Ｅ Ｇ Ｆ） 的表达水平（ Ｆ Ｉ） 及其与肿瘤微血管密度（ Ｍ Ｄ） ，细胞增殖活性（ Ｐ Ｉ） 和淋巴结转移的关系。方法 应用流式细胞术和免疫组织化学技术对５３ 例手术标本进行检测，进行单参数分析和免疫荧光显微镜观察。结果 肺癌组织中 Ｖ Ｅ Ｇ Ｆ 高表达， Ｖ Ｅ Ｇ Ｆ表达阳性肿瘤中， Ｍ Ｄ 和 Ｐ Ｉ明显增高（ Ｐ＜ ００５ 和 Ｐ＜ ００１） ， Ｆ Ｉ和 Ｍ Ｄ、 Ｐ Ｉ显著正相关（ Ｐ＜００１） 。 Ｍ Ｄ 和 Ｐ Ｉ无相关性（ Ｐ＞ ００５） 。 Ｖ Ｅ Ｇ Ｆ 表达水平在淋巴结转移组肿瘤中明显增高（ Ｐ＜ ００１） ，同侧纵隔淋巴结（ Ｎ２） 转移组高于局部淋巴结（ Ｎ１） 转移组（ Ｐ＜ ００５） ，淋巴结转移灶明显高于原发灶（ Ｐ＜ ００１） ， Ｌｏｇｉｓｔｉｃ 回归显示随 Ｖ Ｅ Ｇ Ｆ表达水平增加，淋巴结转移的危险度显著增大（ Ｐ＜ ００１） 。结论 Ｖ Ｅ Ｇ Ｆ在肺癌生长和转移过程中发挥重要作用。

Objective To study the expression levels of vascular endothelial growth factor (VEGF) and its relation with intratumoral microvessel density(MD), cellular proliferation activity(PI), and lymph node metastasis of primary lung cancer Methods Surgical samples of 53 patients with primary lung cancer were measured The VEGF was immunofluorescence stained with VEGF polyclonal antibody The propidium iodide was used to stain cellular DNA VEGF expression levels and DNA content were analyzed quantitatively using flow cytometry Intratumoral microvessel density was detected by factor VIII polyclonal antibody using immunohistochemistry Results VEGF expression levels (FI) were high in lung cancer (1 3±0 6,positive ratio 73 58%) The cellular proliferation activity (PI) in VEGF positive tumors was significantly higher than that in VEGF negative tumors (29 8%±9 2 %vs 18 7%±6 5%, P <0 01 ) The intratumoral microvessel density (MD) in VEGF positive tumors was higher than that in VEGF negative tumors (19 4±11 3 vs 12 3±8 0, P <0 05 ) No association was found between the MD and PI ( r = 0 169, P >0 05) The VEGF expression levels in lung cancer tissues with lymph node metastasis ( n =33) were significantly higher than that in those without lymph node metastasis ( n = 20 ) (1 5±0 6 vs 1 0±0 5, P <0 01 ) Conclusion VEGF plays an important role in the growth and metastasis of lung cancer