气相色谱法测定麝香草酚中的有机溶剂残留量

Determination of Residual Solvents in Thymol by Gas Chromatography

目的建立了麝香草酚中有机残留溶剂异丙醇的气相色谱测定法。方法采用DB-毛细管色谱柱（30m×0．25mm×1μm），以氮气为载气，FID检测器，进样口温度为250℃，检测器温度为300℃。采用程序升温，柱温：40℃，保持8min，然后以30℃／min的速率升至200℃，测定残留溶剂异丙醇的含量。结果线性范围为9．8～196．6μg／ml（r=0．9997）；方法的检出限（S／N=3）为1．5×10^-3平均回收率为98．1％，RSD为1．9％。结论本方法简便，结果准确，重现性好，可用于麝香草酚中残留溶剂异丙醇的测定。

Objective To established a method for the determination of isopropanol in thymol by capillary gas chromatography. Method The DB-1 （30 m ×0.25 mm × 1 μm） was used as capillary column. The nitrogen was used as the carrier gas, and FID as detector. The injector temperature was 250℃, and the detector temperature was 300℃. The column temperature program was initially 40℃（ hold for 8 min） and increased at the rate of 30℃/min to 240℃. The quantification of isopropanol was calculated by external standard method. Results The standard curves linear range was 9.8 -196.6 μg/ml（ r =0. 999 7）. The limits of detection was 1.5 × 10-3 μg （S/N = 3 ）. The average recovery was 98. 1%, and RSD is 1.9%. Conclusion The method is accurate, simple, and repeatable. It can be u.sed to determine the organic solvent residue of isopropanol in Thymol.