姜黄素对急性髓性白血病细胞HL-60增殖和凋亡的影响

Effects of Curcumin on Proliferation and Apoptosis in Acute Myeloid Leukemic Cells HL 60

为了探讨姜黄素对白血病细胞的杀灭能力， 运用细胞培养、流式细胞仪和末端ＴｄＴ酶标记技术系统研究其药理作用和方式。结果发现， 姜黄素能选择性抑制急性髓性白血病细胞ＨＬ－６０ 的增殖， 呈时间与剂量依赖曲线， 姜黄素体外抑制ＨＬ－６０ 细胞２４ ｈ 达到最大峰值。亚Ｇ１ 凋亡峰出现在１２ ｈ 后，并随时间延长而逐步增加，２４ ｈ 可达３４．４％ 。用末端ＴｄＴ酶标记法进一步证实凋亡的细胞数在同一时相可达到４１％ 。结果提示： 姜黄素有明确的抗白血病细胞增殖的能力， 其作用机理是诱导细胞凋亡， 体外浓度和作用时间可作为其进一步临床运用的参考。

To investigate the curcumin killing leukemic cells in vitro, the pharmacological effects of curcumin were studied by using cellular culture, flow cytometry and TUNEL methods The results showed that curcumin selectively inhibited proliferation of acute myeloid leukemic(AML) HL 60 cell lines in a dose and time dependent manner The growth inhibition rate was gradually increased and reached the peak at concentration of 25 μmol/L curcumin at 24 h The sub-G1 peak appeared after 12 h treatment and was increased to 34 4% at 24 h The TUNEL method further certified that apoptotic cells reached 41% at the same phase It was suggested that curcumin possesses obvious potent of anti leukemic cell proliferation, which is contributed to the induction of HL 60 cells apoptosis The concentration and action time of curcumin in vitro provide some reference for clinical use