UF-100尿沉渣分析仪、干化学分析仪与镜检联合检测的应用

Combined use of UF-100 automated urinalysis analyzer,dry chemistry analyzer and microscopy for urinary detection

目的:探讨UF-100尿沉渣分析仪、干化学分析仪与镜检3种方法联合检测管形、尿蛋白的重要性。方法:2 771例尿标本分别用UF-100尿沉渣分析仪、干化学分析仪和Olympus显微镜检测管形、尿蛋白,并且对3种方法的结果进行比较分析。结果:3种方法比较:(1)沉渣法管形阴性、干化学法蛋白阴性、镜检法管形阴性占45.87%;(2)沉渣法管形阳性、干化学法蛋白阳性、镜检法管形阳性占14.69%;(3)沉渣法管形阴性、干化学法蛋白阳性、镜检法管形阴性占5.4%;(4)沉渣法管形阴性、干化学法蛋白阳性、镜检法管形阳性占0.73%;(5)沉渣法管形阳性、干化学法蛋白阳性、镜检法管形阴性占23.42%;(6)沉渣法管形阳性、干化学法蛋白阴性、镜检法管形阳性占9.82%;(7)沉渣法管形阴性、干化学法蛋白阴性、镜检法管形阳性占0.07%。3种方法检测结果一致的占60.56%。沉渣法假阳性为23.42%,假阴性为0.79%;干化学法假阳性为24.5%;假阴性为9.89%。结论:由于尿标本成分复杂,尿沉渣分析仪法、干化学分析仪法均存在假阳性和假阳性的现象。为保证结果的可靠性,为临床提供可靠参考依据。首先用尿沉渣分析仪法、干化学分析仪法做筛选,如均阴性可以发报告,若有任何1项结果异常要镜检后才可发报告。

Objective： To show the importance of combined use of UF-100 automated urinalysis analyzer,dry chemistry analyzer and microscopy for detection of tubular cells and proteins in urine samples.Methods： A total of 2771 urine samples were detected with UF-100 automated urinalysis analyzer,dry chemistry analyzer and microscopy;respectively,and efficiency of these three methods were shown.Results：（1） 45.8% samples were shown as negative for tubular cells by UF-100 automated urinalysis analyzer,negative for protein by dry chemistry analyzer, and negative for tubular cells under microscopy： respectively. （2） 14.69% samples were shown as positive for tubular cells by UF-100 automated urinalysis analyzer, positive for protein by dry chemistry analyzer, and positive for tubular cells under microscopy; respectively. （3） 5.4% samples were shown as negative for tubular cells by UF-100 automated urinalysis analyzer, positive for protein by dry chemistry analyzer, and negative for tubular cells under mlcroscopy： respectively. （4） 0.73% samples were shown as negative for tubular cells by UF-100 automated urinalysis analyzer, positive for protein by dry chemistry analyzer, and posiuve for tubular cells under mmroscopy： respectively. （5） 23.42% samples were shown as positive for tubular cells by UF-100 automated urinalysis analyzer, positive for protem by dry chemistry analyzer, and negauve for tubular ceils under mmroscopy： respectively. （6） 9.82% samples were shown as negative for tubular cells by UF-100 automated urinalysis analyzer, positive for protein by dry chemistry analyzer, and negative for tubular cells under mmroscopy~ respectively. （7） 0.07% samples were shown as negative for tubular ceils by UF-100 automated urinalysis analyzer, negative for protein by dry chemistry analyzer, and positwe for tubular cells under mmroscopy; respectively. Detection results were unanimous for 60. 56% samples, and false positive and negative results revealed by UF-100 automated urinalysis analyzer, dry chemistry analyzer were 23.42% vs 24.5% and0.79 vs 9.89%. Conclusions： Due to complex composition of urine samples, both UF-100 automated urinalysis analyzer and dry chemistry analyzer may produce false results. Itrs highly recommended that urine samples should be detected by both UF 100 automated urinalysis analyzer and dry chemistry analyzer, further detection under microscopy is necessary in case one of them show positive result.