马铃薯干腐病主要致病菌DNA提取方法比较

Comparison of the Major Pathogen DNA Extraction Methods of Potato Dry Rots

马铃薯干腐病是由镰刀菌导致的马铃薯窖储真菌病害之一,获得高质量镰刀菌的DNA是建立马铃薯干腐病分子检测的前提。本试验采用改良SDS法和2×CTAB法对黑龙江省马铃薯干腐病五种镰刀菌的DNA提取方法进行了比较。结果表明,在腐皮镰刀菌(Fusarium solani(Mart.))和拟丝孢镰刀菌(Fusarium trichothecioides Wollenw)的DNA提取过程中,改良SDS法优于2×CTAB,获得的DNA含量较高、条带较亮、纯度较高、完整性较好,而对于燕麦镰刀菌(Fusarium avenaceum(Corda&Fr.)Sacc)、接骨木镰刀(Fusarium sambucinum Fuckel)和拟枝镰刀菌(Fusarium sporotrioides Sherb)的DNA提取两种方法差异不大,但获得的DNA都可以很好的应用到后面的分子实验过程中,此试验获得的结果可以为马铃薯干腐病致病菌的分子生物学研究提供基础。

Potato dry rot is one of fungal diseases caused by Fusarium when it was storaged in pit ,while in order to detect this kind of disease in the molecular level,it is necessary to obtain high quality DNA. In this study, through modified SDS method and 2 × CTAB method, we compared five Fusarium DNA extraction methods of potato dry rot in Heilongjiang Province. The results showed that Modified SDS method was better than 2 × CTAB for Fusarium solani （Mart.） and Fusarium trichothecioides Wollenw, the DNA content higher, the band brighter, the purity higher and the integrity better. But, two methods of DNA extraction difference was little for Fusarium avenaceum （Corda . Fr.） Sacc ,Fusarium sambucinum Fuckel, and Fusarium sporotrioides Sherb.However, the acquired DNA could be well applied into molecule experiment. The experiment results obtained could provide some basis for the molecular research of potato dry rot pathogen.