羽毛氨基酸含量测定影响因素分析

Analysis on Affected Factors of Amino Acids Contents Detection for Feather

羽毛角蛋白中氨基酸含量是研究羽毛结构及羽毛降解产品的重要参数之一,建立羽毛中氨基酸测定方法,为进一步优化羽毛降解工艺和深入研究奠定基础,并对羽毛产品质量控制提供技术支持具有重要的意义。笔者利用L-8800氨基酸分析仪,参照GB/T18246—2000（7.1.1.1）饲料中氨基酸的测定方法,针对羽毛氨基酸测定的几个主要影响因素进行分析讨论,比较在110℃水解温度下,不同盐酸浓度、不同水解时间对家禽羽毛氨基酸含量测定的影响。结果表明：盐酸浓度在4~5mol/L时,氨基酸含量最高;随着水解时间的延长,氨基酸总量在水解时间为20h时,达到最大值,随后呈现下降趋势;羽毛不同部位的氨基酸组成检测发现,羽干的氨基酸总量明显高于羽片。改良水解条件后,羽毛氨基酸含量测定值达到91.41%,比常规酸水解高出3.96%。因此,测定羽毛中的氨基酸,在保证羽毛样品均匀性的前提下,建议选择水解条件为盐酸浓度4~5mol/L、水解时间为20h。

The objective of this study is to optimize the condition of feather amino acid detection, which is critical for feather degradation in feed industry. The amino acids contents detection of feather was carried out by L-8800 amino acid analyzer in the accordance with GB/T 18246-2000（7.1.1.1）. Affected factors including HCl concentration and hydrolysis time were analyzed. The results showed measured value rose when HCl concentration rose or prolonged hydrolysis time properly. The measured value rose by 3.96% at optimum condition. Furthermore, the composition of amino acids was different between feather shaft and pinna. The optimum condition for feather amino acid detection was as followed： hydrolyzed by 4-5 mol/L HCl for 20 h.